Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605

Fumiko Taguchi, Masanobu Yamamoto, Mayumi Ohnishi-Kameyama, Masako Iwaki, Mitsuru Yoshida, Tadashi Ishii, Tomoyuki Konishi, Yuki Ichinose

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Abstract

Flagellar motility and its glycosylation are indispensable for the virulence of Pseudomonas syringae pv. tabaci 6605. Six serine residues of the flagellin protein at positions 143, 164, 176, 183, 193 and 201 are glycosylated, and the glycan structure at 201 was determined to consist of a trisaccharide of two L-rhamnosyl residues and a modified 4-amino-4,6-dideoxyglucosyl (viosamine) residue. To investigate the glycan structures attached to the other serine residues and to identify the glycans important for virulence, Ser/Ala-substituted mutants were generated. Six mutant strains that each retained a single glycosylated serine residue were generated by replacing five of the six serine residues with alanine residues. MALDI-TOF mass analysis of flagellin proteins revealed that the major component of each glycan was a trisaccharide basically similar to that at position 201, but with heterogeneity in glycoform distribution. Swarming motility and amounts of acylhomoserine lactones (AHLs) as quorum-sensing signal molecules were significantly reduced, especially in the S143-5S/A, S164-5S/A and S201-5S/A mutants, whereas tolerance to antibiotics was increased in these three mutants. All the mutants showed lower ability to cause disease on host tobacco plants. These results supported our previous finding that glycosylation of the most externally located sites on the surface of the flagellin molecule, such as S176 and S183, is required for virulence in P. syringae pv. tabaci 6605. Furthermore, it is speculated that flagellum-dependent motility might be correlated with quorum sensing and antibiotic resistance.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalMicrobiology
Volume156
Issue number1
DOIs
Publication statusPublished - 2010

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Pseudomonas syringae
Flagellin
Glycosylation
Serine
Polysaccharides
Virulence
Trisaccharides
Quorum Sensing
Flagella
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Lactones
Microbial Drug Resistance
Alanine
Tobacco
Proteins
Anti-Bacterial Agents

ASJC Scopus subject areas

  • Microbiology

Cite this

Taguchi, F., Yamamoto, M., Ohnishi-Kameyama, M., Iwaki, M., Yoshida, M., Ishii, T., ... Ichinose, Y. (2010). Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605. Microbiology, 156(1), 72-80. https://doi.org/10.1099/mic.0.030700-0

Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605. / Taguchi, Fumiko; Yamamoto, Masanobu; Ohnishi-Kameyama, Mayumi; Iwaki, Masako; Yoshida, Mitsuru; Ishii, Tadashi; Konishi, Tomoyuki; Ichinose, Yuki.

In: Microbiology, Vol. 156, No. 1, 2010, p. 72-80.

Research output: Contribution to journalArticle

Taguchi, F, Yamamoto, M, Ohnishi-Kameyama, M, Iwaki, M, Yoshida, M, Ishii, T, Konishi, T & Ichinose, Y 2010, 'Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605', Microbiology, vol. 156, no. 1, pp. 72-80. https://doi.org/10.1099/mic.0.030700-0
Taguchi F, Yamamoto M, Ohnishi-Kameyama M, Iwaki M, Yoshida M, Ishii T et al. Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605. Microbiology. 2010;156(1):72-80. https://doi.org/10.1099/mic.0.030700-0
Taguchi, Fumiko ; Yamamoto, Masanobu ; Ohnishi-Kameyama, Mayumi ; Iwaki, Masako ; Yoshida, Mitsuru ; Ishii, Tadashi ; Konishi, Tomoyuki ; Ichinose, Yuki. / Defects in flagellin glycosylation affect the virulence of Pseudomonas syringae pv. tabaci 6605. In: Microbiology. 2010 ; Vol. 156, No. 1. pp. 72-80.
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