TY - JOUR
T1 - Defects in d-rhamnosyl residue biosynthetic genes affect lipopolysaccharide structure, motility, and cell-surface hydrophobicity in pseudomonas syringae pathovar glycinea race 4
AU - Chiku, Kazuhiro
AU - Tsunemi, Kazuhiko
AU - Yamamoto, Masanobu
AU - Mayumi, Ohnishi Kameyama
AU - Yoshida, Mitsuru
AU - Ishii, Tadashi
AU - Taguchi, Fumiko
AU - Iwaki, Masako
AU - Ichinose, Yuki
AU - Ono, Hiroshi
N1 - Funding Information:
We thank Prof A. Collmer of Cornell University (Ithaca, NY) for providing P. syringae pv. glycinea race 4. This study was supported in part by Technology of Japan and the Program for the Promotion of Basic Research Activities for Innovative Bioscience (PROBRAIN).
PY - 2013
Y1 - 2013
N2 - D-rhamnose (D-Rha) residue is a major component of lipopolysaccharides (LPSs) in strains of the phytopathogen Pseudomonas syringae pathovar glycinea. To investigate the effects of a deficiency in GDP-D-rhamnose biosynthetic genes on LPS structure and pathogenicity, we generated three mutants defective in D-Rha biosynthetic genes, encoding proteins GDP-D-mannose 4,6-dehydratase (GMD), GDP-4-keto-6-deoxy-D-mannose reductase (RMD), and a putative Δ-D-rhamnosyltransferase (WbpZ) in P. syringae pv. glycinea race 4. The Δgmd, Δrmd, and ΔwbpZ mutants had a reduced O-antigen polysaccharide consisting of D-Rha residues as compared with the wild type (WT). The swarming motility of the Δgmd, Δrmd, and ΔwbpZ mutant strains decreased and hydrophobicity and adhesion ability increased as compared with WT. Although the mutants had truncated O-antigen polysaccharides, and altered surface properties, they showed virulence to soybean, as WT did.
AB - D-rhamnose (D-Rha) residue is a major component of lipopolysaccharides (LPSs) in strains of the phytopathogen Pseudomonas syringae pathovar glycinea. To investigate the effects of a deficiency in GDP-D-rhamnose biosynthetic genes on LPS structure and pathogenicity, we generated three mutants defective in D-Rha biosynthetic genes, encoding proteins GDP-D-mannose 4,6-dehydratase (GMD), GDP-4-keto-6-deoxy-D-mannose reductase (RMD), and a putative Δ-D-rhamnosyltransferase (WbpZ) in P. syringae pv. glycinea race 4. The Δgmd, Δrmd, and ΔwbpZ mutants had a reduced O-antigen polysaccharide consisting of D-Rha residues as compared with the wild type (WT). The swarming motility of the Δgmd, Δrmd, and ΔwbpZ mutant strains decreased and hydrophobicity and adhesion ability increased as compared with WT. Although the mutants had truncated O-antigen polysaccharides, and altered surface properties, they showed virulence to soybean, as WT did.
KW - D-rhamnose
KW - Lipopolysaccharide
KW - Mutation
KW - O-antigen polysaccharide
KW - Pseudomonas syringae
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U2 - 10.1271/bbb.120736
DO - 10.1271/bbb.120736
M3 - Article
C2 - 23470736
AN - SCOPUS:84876370329
VL - 77
SP - 505
EP - 510
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 3
ER -