Defects in d-rhamnosyl residue biosynthetic genes affect lipopolysaccharide structure, motility, and cell-surface hydrophobicity in pseudomonas syringae pathovar glycinea race 4

Kazuhiro Chiku, Kazuhiko Tsunemi, Masanobu Yamamoto, Ohnishi Kameyama Mayumi, Mitsuru Yoshida, Tadashi Ishii, Fumiko Taguchi, Masako Iwaki, Yuki Ichinose, Hiroshi Ono

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

D-rhamnose (D-Rha) residue is a major component of lipopolysaccharides (LPSs) in strains of the phytopathogen Pseudomonas syringae pathovar glycinea. To investigate the effects of a deficiency in GDP-D-rhamnose biosynthetic genes on LPS structure and pathogenicity, we generated three mutants defective in D-Rha biosynthetic genes, encoding proteins GDP-D-mannose 4,6-dehydratase (GMD), GDP-4-keto-6-deoxy-D-mannose reductase (RMD), and a putative Δ-D-rhamnosyltransferase (WbpZ) in P. syringae pv. glycinea race 4. The Δgmd, Δrmd, and ΔwbpZ mutants had a reduced O-antigen polysaccharide consisting of D-Rha residues as compared with the wild type (WT). The swarming motility of the Δgmd, Δrmd, and ΔwbpZ mutant strains decreased and hydrophobicity and adhesion ability increased as compared with WT. Although the mutants had truncated O-antigen polysaccharides, and altered surface properties, they showed virulence to soybean, as WT did.

Original languageEnglish
Pages (from-to)505-510
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume77
Issue number3
DOIs
Publication statusPublished - Apr 24 2013

Keywords

  • D-rhamnose
  • Lipopolysaccharide
  • Mutation
  • O-antigen polysaccharide
  • Pseudomonas syringae

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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