Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease

Md Fakruddin, Fan Yan Wei, Takeo Suzuki, Kana Asano, Takashi Kaieda, Akiko Omori, Ryoma Izumi, Atsushi Fujimura, Taku Kaitsuka, Keishi Miyata, Kimi Araki, Yuichi Oike, Luca Scorrano, Tsutomu Suzuki, Kazuhito Tomizawa

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A subset of mitochondrial tRNAs (mt-tRNAs) contains taurine-derived modifications at 34U of the anticodon. Loss of taurine modification has been linked to the development of mitochondrial diseases, but the molecular mechanism is still unclear. Here, we showed that taurine modification is catalyzed by mitochondrial optimization 1 (Mto1) in mammals. Mto1 deficiency severely impaired mitochondrial translation and respiratory activity. Moreover, Mto1-deficient cells exhibited abnormal mitochondrial morphology owing to aberrant trafficking of nuclear DNA-encoded mitochondrial proteins, including Opa1. The mistargeted proteins were aggregated and misfolded in the cytoplasm, which induced cytotoxic unfolded protein response. Importantly, application of chemical chaperones successfully suppressed cytotoxicity by reducing protein misfolding and increasing functional mitochondrial proteins in Mto1-deficient cells and mice. Thus, our results demonstrate the essential role of taurine modification in mitochondrial translation and reveal an intrinsic protein homeostasis network between the mitochondria and cytosol, which has therapeutic potential for mitochondrial diseases. Taurine modification of mitochondrial tRNA is associated with mitochondrial disease. Fakruddin et al. find that taurine modification is indispensable for mitochondrial protein translation. The authors also find that deficiency of taurine modification impairs a mitochondrial-cytosolic proteostatic network through an Opa1-dependent mechanism and demonstrate the therapeutic potential of chemical chaperones.

Original languageEnglish
Pages (from-to)482-496
Number of pages15
JournalCell Reports
Volume22
Issue number2
DOIs
Publication statusPublished - Jan 1 2018
Externally publishedYes

Fingerprint

Mitochondrial Diseases
Taurine
Transfer RNA
Mitochondrial Proteins
Proteins
Anticodon
Unfolded Protein Response
Mitochondria
Mammals
Protein Biosynthesis
Cytotoxicity
Cytosol
Cytoplasm
Homeostasis
DNA
Therapeutics

Keywords

  • mitochondria
  • modification
  • Opa1
  • taurine
  • tRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease. / Fakruddin, Md; Wei, Fan Yan; Suzuki, Takeo; Asano, Kana; Kaieda, Takashi; Omori, Akiko; Izumi, Ryoma; Fujimura, Atsushi; Kaitsuka, Taku; Miyata, Keishi; Araki, Kimi; Oike, Yuichi; Scorrano, Luca; Suzuki, Tsutomu; Tomizawa, Kazuhito.

In: Cell Reports, Vol. 22, No. 2, 01.01.2018, p. 482-496.

Research output: Contribution to journalArticle

Fakruddin, M, Wei, FY, Suzuki, T, Asano, K, Kaieda, T, Omori, A, Izumi, R, Fujimura, A, Kaitsuka, T, Miyata, K, Araki, K, Oike, Y, Scorrano, L, Suzuki, T & Tomizawa, K 2018, 'Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease', Cell Reports, vol. 22, no. 2, pp. 482-496. https://doi.org/10.1016/j.celrep.2017.12.051
Fakruddin, Md ; Wei, Fan Yan ; Suzuki, Takeo ; Asano, Kana ; Kaieda, Takashi ; Omori, Akiko ; Izumi, Ryoma ; Fujimura, Atsushi ; Kaitsuka, Taku ; Miyata, Keishi ; Araki, Kimi ; Oike, Yuichi ; Scorrano, Luca ; Suzuki, Tsutomu ; Tomizawa, Kazuhito. / Defective Mitochondrial tRNA Taurine Modification Activates Global Proteostress and Leads to Mitochondrial Disease. In: Cell Reports. 2018 ; Vol. 22, No. 2. pp. 482-496.
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