Defective active silicon uptake affects some components of rice resistance to brown spot

Leandro J. Dallagnol, Fabrício Á Rodrigues, Mateus V B Mielli, Jian Feng Ma, Lawrence E. Datnoff

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Rice is known to accumulate high amounts of silicon (Si) in plant tissue, which helps to decrease the intensity of many economically important rice diseases. Among these diseases, brown spot, caused by the fungus Bipolaris oryzae, is one of the most devastating because it negatively affects yield and grain quality. This study aimed to evaluate the importance of active root Si uptake in rice for controlling brown spot development. Some components of host resistance were evaluated in a rice mutant, low silicon 1 (lsi1), defective in active Si uptake, and its wild-type counterpart (cv. Oochikara). Plants were inoculated with B. oryzae after growing for 35 days in a hydroponic culture amended with 0 or 2 mMol Si. The components of host resistance evaluated were incubation period (IP), relative infection efficiency (RIE), area under brown spot progress curve (AUBSPC), final lesion size (FLS), rate of lesion expansion (r), and area under lesion expansion progress curve (AULEPC). Si content from both Oochikara and lsi1 in the +Si treatment increased in leaf tissue by 219 and 178%, respectively, over the nonamended controls. Plants from Oochikara had 112% more Si in leaf tissue than plants from lsi1. The IP of brown spot from Oochikara increased ≈6 h in the presence of Si and the RIE, AUBSPC, FLS, r, and AULEPC were significantly reduced by 65, 75, 33, 36, and 35%, respectively. In the presence of Si, the IP increased 3 h for lsi1 but the RIE, AUBSPC, FLS, r, and AULEPC were reduced by only 40, 50, 12, 21, and 12%, respectively. The correlation between Si leaf content and IP was significantly positive but Si content was negatively correlated with RIE, AUBSPC, FLS, r, and AULEPC. Single degree-of-freedom contrasts showed that Oochikara and lsi1 supplied with Si were significantly different from those not supplied with Si for all components of resistance evaluated. This result showed that a reduced Si content in tissues of plants from lsi1 dramatically affected its basal level of resistance to brown spot, suggesting that a minimum Si concentration is needed. Consequently, the results of this study emphasized the importance of an active root Si uptake system for an increase in rice resistance to brown spot.

Original languageEnglish
Pages (from-to)116-121
Number of pages6
JournalPhytopathology
Volume99
Issue number1
DOIs
Publication statusPublished - Jan 2009

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silicon
rice
lesions (plant)
plant tissues
infection
beriberi
leaves

Keywords

  • Foliar disease
  • Oryza sativa

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Defective active silicon uptake affects some components of rice resistance to brown spot. / Dallagnol, Leandro J.; Rodrigues, Fabrício Á; Mielli, Mateus V B; Ma, Jian Feng; Datnoff, Lawrence E.

In: Phytopathology, Vol. 99, No. 1, 01.2009, p. 116-121.

Research output: Contribution to journalArticle

Dallagnol, Leandro J. ; Rodrigues, Fabrício Á ; Mielli, Mateus V B ; Ma, Jian Feng ; Datnoff, Lawrence E. / Defective active silicon uptake affects some components of rice resistance to brown spot. In: Phytopathology. 2009 ; Vol. 99, No. 1. pp. 116-121.
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