Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans: Analysis of clinical strains isolated from oral cavities

N. Taniguchi, K. Nakano, R. Nomura, Shuhei Naka, A. Kojima, Michiyo Nakano, T. Ooshima

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.

Original languageEnglish
Pages (from-to)410-416
Number of pages7
JournalArchives of Oral Biology
Volume55
Issue number6
DOIs
Publication statusPublished - Jun 2010
Externally publishedYes

Fingerprint

Glucosyltransferases
Streptococcus mutans
Platelet Aggregation
Mouth
Platelet-Rich Plasma
Endocarditis
Western Blotting
Dental Caries
Virulence
Immune Sera
Blood Platelets
Cell Count
Polymerase Chain Reaction

Keywords

  • Clinical isolates
  • Glucosyltransferases
  • Platelet aggregation property
  • Streptococcus mutans

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Cell Biology
  • Dentistry(all)

Cite this

Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans : Analysis of clinical strains isolated from oral cavities. / Taniguchi, N.; Nakano, K.; Nomura, R.; Naka, Shuhei; Kojima, A.; Nakano, Michiyo; Ooshima, T.

In: Archives of Oral Biology, Vol. 55, No. 6, 06.2010, p. 410-416.

Research output: Contribution to journalArticle

@article{42377d8f1abc49e4aa68915e1c9c3be6,
title = "Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans: Analysis of clinical strains isolated from oral cavities",
abstract = "Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as {"}GTFBC-fusion{"} strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.",
keywords = "Clinical isolates, Glucosyltransferases, Platelet aggregation property, Streptococcus mutans",
author = "N. Taniguchi and K. Nakano and R. Nomura and Shuhei Naka and A. Kojima and Michiyo Nakano and T. Ooshima",
year = "2010",
month = "6",
doi = "10.1016/j.archoralbio.2010.03.017",
language = "English",
volume = "55",
pages = "410--416",
journal = "Archives of Oral Biology",
issn = "0003-9969",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans

T2 - Analysis of clinical strains isolated from oral cavities

AU - Taniguchi, N.

AU - Nakano, K.

AU - Nomura, R.

AU - Naka, Shuhei

AU - Kojima, A.

AU - Nakano, Michiyo

AU - Ooshima, T.

PY - 2010/6

Y1 - 2010/6

N2 - Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.

AB - Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.

KW - Clinical isolates

KW - Glucosyltransferases

KW - Platelet aggregation property

KW - Streptococcus mutans

UR - http://www.scopus.com/inward/record.url?scp=77955420812&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955420812&partnerID=8YFLogxK

U2 - 10.1016/j.archoralbio.2010.03.017

DO - 10.1016/j.archoralbio.2010.03.017

M3 - Article

C2 - 20398892

AN - SCOPUS:77955420812

VL - 55

SP - 410

EP - 416

JO - Archives of Oral Biology

JF - Archives of Oral Biology

SN - 0003-9969

IS - 6

ER -