Abstract
Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.
| Original language | English |
|---|---|
| Pages (from-to) | 410-416 |
| Number of pages | 7 |
| Journal | Archives of Oral Biology |
| Volume | 55 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Jun 2010 |
| Externally published | Yes |
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Keywords
- Clinical isolates
- Glucosyltransferases
- Platelet aggregation property
- Streptococcus mutans
ASJC Scopus subject areas
- Otorhinolaryngology
- Cell Biology
- Dentistry(all)
Cite this
Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans : Analysis of clinical strains isolated from oral cavities. / Taniguchi, N.; Nakano, K.; Nomura, R.; Naka, Shuhei; Kojima, A.; Nakano, Michiyo; Ooshima, T.
In: Archives of Oral Biology, Vol. 55, No. 6, 06.2010, p. 410-416.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Defect of glucosyltransferases reduces platelet aggregation activity of Streptococcus mutans
T2 - Analysis of clinical strains isolated from oral cavities
AU - Taniguchi, N.
AU - Nakano, K.
AU - Nomura, R.
AU - Naka, Shuhei
AU - Kojima, A.
AU - Nakano, Michiyo
AU - Ooshima, T.
PY - 2010/6
Y1 - 2010/6
N2 - Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.
AB - Objective: Streptococcus mutans is a major pathogen of dental caries and occasionally isolated from the blood of patients with infective endocarditis, though the association of its cell-surface glucosyltransferases (GTFB, GTFC, and GTFD) with pathogenicity for infective endocarditis remains to be elucidated. In this study, we investigated the contribution of S. mutans GTFs to platelet aggregation and analysed GTF expression profiles in a large number of clinical oral isolates. Design: The platelet aggregation properties of GTF-defective isogenic mutant strains constructed from S. mutans reference strain MT8148 were evaluated using whole blood and platelet-rich plasma (PRP) taken from mice, as well as human PRP. In addition, GTF expression profiles for 396 S. mutans strains isolated from the oral cavities of 396 subjects were analysed by western blotting using antisera specific for each GTF. Results: The platelet aggregation activities of the GTF-defective isogenic mutants were significantly lower than that of MT8148 when added to a large number of cells. Western blotting revealed no strains without GTF expression, though six strains had alterations of GTFB and GTFC as compared to MT8148. PCR analyses indicated that the gtfB-gtfC region length was approximately 4.5 kb shorter in those strains as compared to MT8148. These were designated as "GTFBC-fusion" strains and they demonstrated lower levels of platelet aggregation. Conclusions: Our findings indicate that GTFs are associated with platelet aggregation. Although the clinical detection frequency of S. mutans strains with altered expressions is extremely low, GTFBC-fusion strains have activities similar to GTF-defective mutant strains.
KW - Clinical isolates
KW - Glucosyltransferases
KW - Platelet aggregation property
KW - Streptococcus mutans
UR - http://www.scopus.com/inward/record.url?scp=77955420812&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955420812&partnerID=8YFLogxK
U2 - 10.1016/j.archoralbio.2010.03.017
DO - 10.1016/j.archoralbio.2010.03.017
M3 - Article
C2 - 20398892
AN - SCOPUS:77955420812
VL - 55
SP - 410
EP - 416
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 6
ER -