Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma

Keishi Fujiwara, Toshihiro Higashi, Kazuhiro Nouso, Harushige Nakatsukasa, Yoshiyuki Kobayashi, Masayuki Uemura, Shin Ichiro Nakamura, Shuichiro Sato, Tadashi Hanafusa, Yasuhiro Yumoto, Ichiro Naito, Yasushi Shiratori

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Background and Aim: We analyzed the expression of antigen-processing and antigen-presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)-10 protein, which might act to downregulate expression of antigen-processing and antigen-presenting molecules. Methods: Twenty-eight HCC samples obtained by surgical resection were analyzed for the expression of β2-microglobulin, heat-shock protein (HSP)-70, human leukocyte antigen (HLA) class-I, CD80 (B7-1), CD86 (B7-2) and IL-10 by immunostaining. Results: β2-Microglobulin and HSP-70 were preserved in all samples. In contrast, the expression of HLA class-I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7-1 and B7-2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL-10 protein was expressed in 54% of HCC, no relationship between the expression of IL-10 and downregulation of B7-1, B7-2, and HLA class-I was evident. Conclusion: These findings suggest the potential role of B7 co-stimulatory molecules and HLA class-I molecules in facilitating HCC escape from immune surveillance without the involvement of IL-10.

Original languageEnglish
Pages (from-to)1121-1127
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume19
Issue number10
DOIs
Publication statusPublished - 2004

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B7 Antigens
Major Histocompatibility Complex
Interleukin-10
Hepatocellular Carcinoma
HLA Antigens
HSP70 Heat-Shock Proteins
Neoplasm Grading
Antigen Presentation
Down-Regulation
Antigens
Proteins
Liver
Neoplasms

Keywords

  • Immune tolerance
  • Liver
  • Neoplasm
  • Tumor escape

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma. / Fujiwara, Keishi; Higashi, Toshihiro; Nouso, Kazuhiro; Nakatsukasa, Harushige; Kobayashi, Yoshiyuki; Uemura, Masayuki; Nakamura, Shin Ichiro; Sato, Shuichiro; Hanafusa, Tadashi; Yumoto, Yasuhiro; Naito, Ichiro; Shiratori, Yasushi.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 19, No. 10, 2004, p. 1121-1127.

Research output: Contribution to journalArticle

Fujiwara, K, Higashi, T, Nouso, K, Nakatsukasa, H, Kobayashi, Y, Uemura, M, Nakamura, SI, Sato, S, Hanafusa, T, Yumoto, Y, Naito, I & Shiratori, Y 2004, 'Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma', Journal of Gastroenterology and Hepatology (Australia), vol. 19, no. 10, pp. 1121-1127. https://doi.org/10.1111/j.1440-1746.2004.03467.x
Fujiwara, Keishi ; Higashi, Toshihiro ; Nouso, Kazuhiro ; Nakatsukasa, Harushige ; Kobayashi, Yoshiyuki ; Uemura, Masayuki ; Nakamura, Shin Ichiro ; Sato, Shuichiro ; Hanafusa, Tadashi ; Yumoto, Yasuhiro ; Naito, Ichiro ; Shiratori, Yasushi. / Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma. In: Journal of Gastroenterology and Hepatology (Australia). 2004 ; Vol. 19, No. 10. pp. 1121-1127.
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abstract = "Background and Aim: We analyzed the expression of antigen-processing and antigen-presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)-10 protein, which might act to downregulate expression of antigen-processing and antigen-presenting molecules. Methods: Twenty-eight HCC samples obtained by surgical resection were analyzed for the expression of β2-microglobulin, heat-shock protein (HSP)-70, human leukocyte antigen (HLA) class-I, CD80 (B7-1), CD86 (B7-2) and IL-10 by immunostaining. Results: β2-Microglobulin and HSP-70 were preserved in all samples. In contrast, the expression of HLA class-I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7-1 and B7-2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21{\%} and 36{\%}, respectively). Although IL-10 protein was expressed in 54{\%} of HCC, no relationship between the expression of IL-10 and downregulation of B7-1, B7-2, and HLA class-I was evident. Conclusion: These findings suggest the potential role of B7 co-stimulatory molecules and HLA class-I molecules in facilitating HCC escape from immune surveillance without the involvement of IL-10.",
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T1 - Decreased expression of B7 costimulatory molecules and major histocompatibility complex class-I in human hepatocellular carcinoma

AU - Fujiwara, Keishi

AU - Higashi, Toshihiro

AU - Nouso, Kazuhiro

AU - Nakatsukasa, Harushige

AU - Kobayashi, Yoshiyuki

AU - Uemura, Masayuki

AU - Nakamura, Shin Ichiro

AU - Sato, Shuichiro

AU - Hanafusa, Tadashi

AU - Yumoto, Yasuhiro

AU - Naito, Ichiro

AU - Shiratori, Yasushi

PY - 2004

Y1 - 2004

N2 - Background and Aim: We analyzed the expression of antigen-processing and antigen-presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)-10 protein, which might act to downregulate expression of antigen-processing and antigen-presenting molecules. Methods: Twenty-eight HCC samples obtained by surgical resection were analyzed for the expression of β2-microglobulin, heat-shock protein (HSP)-70, human leukocyte antigen (HLA) class-I, CD80 (B7-1), CD86 (B7-2) and IL-10 by immunostaining. Results: β2-Microglobulin and HSP-70 were preserved in all samples. In contrast, the expression of HLA class-I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7-1 and B7-2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL-10 protein was expressed in 54% of HCC, no relationship between the expression of IL-10 and downregulation of B7-1, B7-2, and HLA class-I was evident. Conclusion: These findings suggest the potential role of B7 co-stimulatory molecules and HLA class-I molecules in facilitating HCC escape from immune surveillance without the involvement of IL-10.

AB - Background and Aim: We analyzed the expression of antigen-processing and antigen-presenting molecules in surgically resected fresh samples of human hepatocellular carcinoma (HCC) tissue to elucidate a mechanism of immune escape. We also examined the expression of interleukin (IL)-10 protein, which might act to downregulate expression of antigen-processing and antigen-presenting molecules. Methods: Twenty-eight HCC samples obtained by surgical resection were analyzed for the expression of β2-microglobulin, heat-shock protein (HSP)-70, human leukocyte antigen (HLA) class-I, CD80 (B7-1), CD86 (B7-2) and IL-10 by immunostaining. Results: β2-Microglobulin and HSP-70 were preserved in all samples. In contrast, the expression of HLA class-I molecules was significantly reduced according to lowering in the histological grading of tumor differentiation (P = 0.024). Furthermore, B7-1 and B7-2 expression was reduced in tumor cells compared with corresponding areas of liver tissue without malignant involvement irrespective of the histological grading of tumors (21% and 36%, respectively). Although IL-10 protein was expressed in 54% of HCC, no relationship between the expression of IL-10 and downregulation of B7-1, B7-2, and HLA class-I was evident. Conclusion: These findings suggest the potential role of B7 co-stimulatory molecules and HLA class-I molecules in facilitating HCC escape from immune surveillance without the involvement of IL-10.

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KW - Neoplasm

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