Decreased depressor response mediated by calcitonin gene-related peptide (CGRP)-containing vasodilator nerves to spinal cord stimulation and levels of CGRP mRNA of the dorsal root ganglia in spontaneously hypertensive rats

H. Kawasaki, Y. Nuki, N. Yamaga, Yuji Kurosaki, T. Taguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The depressor response to electrical stimulation of the spinal cord and the level of calcitonin gene-related peptide (CGRP) mRNA in the dorsal root ganglion (DRG) in the spontaneously hypertensive rat (SHR) was compared with the normotensive Wistar Kyoto rat (WKY) and Wistar rat (WR). The animals were pithed by inserting a stainless-steel rod into the spinal cord. Pithed rats were treated with hexamethonium (2 mg/kg/min i.v.) to block autonomic outflow, and mean arterial blood pressure (MBP) was maintained at approximately 100 mmHg with continuous infusion of methoxamine (10 to 15 μg/kg/min i.v.). Electrical stimulation (2 and 4 Hz for 30 s) of the lower thoracic spinal cord (T9-12) via the pithing rod caused a frequency-dependent depressor response without a change in heart rate. The depressor response to spinal cord stimulation was significantly smaller in SHR than in WKY and WR. Long-term treatment of 8 week-old SHR with captopril (0.1% in drinking water) for 7 weeks restored the reduced depressor response to spinal cord stimulation. The level of CGRP mRNA in DRG of SHR was significantly lower than that in WKY. These results suggest that the function of CGRP-containing nerves from the spinal cord decreases in SHR and captopril treatment prevents its reduction.

Original languageEnglish
Pages (from-to)693-699
Number of pages7
JournalHypertension Research
Volume23
Issue number6
Publication statusPublished - 2000

Fingerprint

Spinal Cord Stimulation
Calcitonin Gene-Related Peptide
Spinal Ganglia
Inbred SHR Rats
Vasodilator Agents
Inbred WKY Rats
Messenger RNA
Spinal Cord
Captopril
Electric Stimulation
Wistar Rats
Arterial Pressure
Methoxamine
Hexamethonium
Stainless Steel
Drinking Water
Thorax
Heart Rate
Therapeutics

Keywords

  • Calcitonin gene-related peptide (CGRP) vasodilator nerve
  • CGRP mRNA
  • Depressor response
  • Pithed rat
  • SHR
  • Spinal cord stimulation

ASJC Scopus subject areas

  • Internal Medicine

Cite this

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title = "Decreased depressor response mediated by calcitonin gene-related peptide (CGRP)-containing vasodilator nerves to spinal cord stimulation and levels of CGRP mRNA of the dorsal root ganglia in spontaneously hypertensive rats",
abstract = "The depressor response to electrical stimulation of the spinal cord and the level of calcitonin gene-related peptide (CGRP) mRNA in the dorsal root ganglion (DRG) in the spontaneously hypertensive rat (SHR) was compared with the normotensive Wistar Kyoto rat (WKY) and Wistar rat (WR). The animals were pithed by inserting a stainless-steel rod into the spinal cord. Pithed rats were treated with hexamethonium (2 mg/kg/min i.v.) to block autonomic outflow, and mean arterial blood pressure (MBP) was maintained at approximately 100 mmHg with continuous infusion of methoxamine (10 to 15 μg/kg/min i.v.). Electrical stimulation (2 and 4 Hz for 30 s) of the lower thoracic spinal cord (T9-12) via the pithing rod caused a frequency-dependent depressor response without a change in heart rate. The depressor response to spinal cord stimulation was significantly smaller in SHR than in WKY and WR. Long-term treatment of 8 week-old SHR with captopril (0.1{\%} in drinking water) for 7 weeks restored the reduced depressor response to spinal cord stimulation. The level of CGRP mRNA in DRG of SHR was significantly lower than that in WKY. These results suggest that the function of CGRP-containing nerves from the spinal cord decreases in SHR and captopril treatment prevents its reduction.",
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author = "H. Kawasaki and Y. Nuki and N. Yamaga and Yuji Kurosaki and T. Taguchi",
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T1 - Decreased depressor response mediated by calcitonin gene-related peptide (CGRP)-containing vasodilator nerves to spinal cord stimulation and levels of CGRP mRNA of the dorsal root ganglia in spontaneously hypertensive rats

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AU - Nuki, Y.

AU - Yamaga, N.

AU - Kurosaki, Yuji

AU - Taguchi, T.

PY - 2000

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