Decrease in the content of cytochrome P450IIE by fasting in liver microsomes of house musk shrew (Suncus murinus)

Hironori Nakura, Susumu Itoh, Hisashi Kusano, Hiroyuki Ishizone, Susumu Imaoka, Yoshihiko Funae, Tsuyoshi Yokoi, Tetsuya Kamataki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The effects of fasting on hepatic cytochrome P450 in the mature male house musk shrew, Suncus murinus (suncus), were studied by Western blot analyses and enzyme assays. The content of P450IIE protein was decreased, by fasting, to 24% of the control level in contrast to the results with rats, in which P450IIE protein was increased to 172% by fasting. These changes reflected on catalytic activities, such as aniline hydroxylase and N-nitrosodimethylamine demethylase activities, which were decreased to about 45% and 28%, respectively, of the control levels by fasting, while in fasting rats, the catalytic activities of these enzymes were 2-3-fold higher than in controls.

Original languageEnglish
Pages (from-to)1907-1910
Number of pages4
JournalBiochemical Pharmacology
Volume43
Issue number9
DOIs
Publication statusPublished - May 8 1992
Externally publishedYes

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Shrews
Level control
Liver Microsomes
Liver
Rats
Fasting
Catalyst activity
Aniline Hydroxylase
Cytochrome P-450 CYP2E1
Enzymes
Cytochrome P-450 Enzyme System
Assays
Proteins
Enzyme Assays
Western Blotting
musk
1,2-dimethylhydrazine demethylase

ASJC Scopus subject areas

  • Pharmacology

Cite this

Decrease in the content of cytochrome P450IIE by fasting in liver microsomes of house musk shrew (Suncus murinus). / Nakura, Hironori; Itoh, Susumu; Kusano, Hisashi; Ishizone, Hiroyuki; Imaoka, Susumu; Funae, Yoshihiko; Yokoi, Tsuyoshi; Kamataki, Tetsuya.

In: Biochemical Pharmacology, Vol. 43, No. 9, 08.05.1992, p. 1907-1910.

Research output: Contribution to journalArticle

Nakura, Hironori ; Itoh, Susumu ; Kusano, Hisashi ; Ishizone, Hiroyuki ; Imaoka, Susumu ; Funae, Yoshihiko ; Yokoi, Tsuyoshi ; Kamataki, Tetsuya. / Decrease in the content of cytochrome P450IIE by fasting in liver microsomes of house musk shrew (Suncus murinus). In: Biochemical Pharmacology. 1992 ; Vol. 43, No. 9. pp. 1907-1910.
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