Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats

Norihiko Obata, Satoshi Mizobuchi, Yoshitaro Itano, Yoshikazu Matsuoka, Ryuji Kaku, Naoto Tomotsuka, Kiyoshi Morita, Hirotaka Kanzaki, Mamoru Oouchida, Masataka Yokoyama

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The mechanism underlying neuropathic pain is still largely unclear. Recently, much attention has been focused on the role of brain-derived neurotrophic factor (BDNF) as a neuromodulator in the spinal cord. We previously reported that the expression of Bdnf exon I mRNA was remarkably up-regulated in the dorsal root ganglion (DRG) neurons with the rat L5 spinal nerve ligation (SNL) model. In the present study, we investigated whether neuropathic pain response would be reduced by the inhibition of the Bdnf exon I in the rat SNL model. We identified the promoter region of exon I and synthesized the decoy ODNs targeting the region. Reverse transcription-polymerase chain reaction analysis confirmed that the decoy ODN treatment reduced SNL-induced Bdnf exon I mRNA up-regulation in ipsilateral L4 and L5 DRGs. Furthermore, post-treatment with the decoy ODNs significantly attenuated SNL-induced tactile allodynia. This study suggested that decoy ODNs targeting the Bdnf exon I might provide a novel analgesic strategy for the treatment of neuropathic pain.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume408
Issue number1
DOIs
Publication statusPublished - Apr 29 2011

Fingerprint

Hyperalgesia
Brain-Derived Neurotrophic Factor
Neuralgia
Spinal Nerves
Rats
Exons
Ligation
Messenger RNA
Diagnosis-Related Groups
Polymerase chain reaction
Spinal Ganglia
Transcription
Genetic Promoter Regions
Reverse Transcription
Neurons
Neurotransmitter Agents
Analgesics
Spinal Cord
Up-Regulation
Therapeutics

Keywords

  • Brain-derived neurotrophic factor
  • Decoy oligodeoxynucleotide
  • Neuropathic pain
  • Spinal nerve ligation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats. / Obata, Norihiko; Mizobuchi, Satoshi; Itano, Yoshitaro; Matsuoka, Yoshikazu; Kaku, Ryuji; Tomotsuka, Naoto; Morita, Kiyoshi; Kanzaki, Hirotaka; Oouchida, Mamoru; Yokoyama, Masataka.

In: Biochemical and Biophysical Research Communications, Vol. 408, No. 1, 29.04.2011, p. 139-144.

Research output: Contribution to journalArticle

Obata, Norihiko ; Mizobuchi, Satoshi ; Itano, Yoshitaro ; Matsuoka, Yoshikazu ; Kaku, Ryuji ; Tomotsuka, Naoto ; Morita, Kiyoshi ; Kanzaki, Hirotaka ; Oouchida, Mamoru ; Yokoyama, Masataka. / Decoy strategy targeting the brain-derived neurotrophic factor exon I to attenuate tactile allodynia in the neuropathic pain model of rats. In: Biochemical and Biophysical Research Communications. 2011 ; Vol. 408, No. 1. pp. 139-144.
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