Decoding property of C5 uridine modification at the wobble position of tRNA anticodon.

Shinya Kurata, Takashi Ohtsuki, Takeshi Wada, Yohei Kirino, Kazuyuki Takai, Kazuhiko Saigo, Kimitsuna Watanabe, Tsutomu Suzuki

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

Post-transcriptional modification at the first (wobble) position of the tRNA anticodon participates in precise decoding of the genetic code. We recently identified a novel taurine-containing modified uridine (tau m5U; 5-taurinomethyluridine) at the wobble position of mammalian mitochondrial tRNAs and found lack of this modification in mutant mitochondrial tRNAs from human pathogenic cells of the mitochondrial encephalomyopathies, investigate molecular pathogenesis of the diseases, decoding activity of wobble uridines with or without C5 modification was measured using E. coli cell-free translation system. It has been revealed that C5 modification has a functional role for stabilizing U:G wobble base pair.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages245-246
Number of pages2
Edition3
Publication statusPublished - 2003
Externally publishedYes

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Anticodon
Uridine
Transfer RNA
Mitochondrial Encephalomyopathies
Genetic Code
Cell-Free System
Taurine
Base Pairing
Escherichia coli

Cite this

Kurata, S., Ohtsuki, T., Wada, T., Kirino, Y., Takai, K., Saigo, K., ... Suzuki, T. (2003). Decoding property of C5 uridine modification at the wobble position of tRNA anticodon. In Nucleic acids research. Supplement (2001) (3 ed., pp. 245-246)

Decoding property of C5 uridine modification at the wobble position of tRNA anticodon. / Kurata, Shinya; Ohtsuki, Takashi; Wada, Takeshi; Kirino, Yohei; Takai, Kazuyuki; Saigo, Kazuhiko; Watanabe, Kimitsuna; Suzuki, Tsutomu.

Nucleic acids research. Supplement (2001). 3. ed. 2003. p. 245-246.

Research output: Chapter in Book/Report/Conference proceedingChapter

Kurata, S, Ohtsuki, T, Wada, T, Kirino, Y, Takai, K, Saigo, K, Watanabe, K & Suzuki, T 2003, Decoding property of C5 uridine modification at the wobble position of tRNA anticodon. in Nucleic acids research. Supplement (2001). 3 edn, pp. 245-246.
Kurata S, Ohtsuki T, Wada T, Kirino Y, Takai K, Saigo K et al. Decoding property of C5 uridine modification at the wobble position of tRNA anticodon. In Nucleic acids research. Supplement (2001). 3 ed. 2003. p. 245-246
Kurata, Shinya ; Ohtsuki, Takashi ; Wada, Takeshi ; Kirino, Yohei ; Takai, Kazuyuki ; Saigo, Kazuhiko ; Watanabe, Kimitsuna ; Suzuki, Tsutomu. / Decoding property of C5 uridine modification at the wobble position of tRNA anticodon. Nucleic acids research. Supplement (2001). 3. ed. 2003. pp. 245-246
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AU - Takai, Kazuyuki

AU - Saigo, Kazuhiko

AU - Watanabe, Kimitsuna

AU - Suzuki, Tsutomu

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