DEC205 mediates local and systemic immune responses to Helicobacter pylori infection in humans

Masahide Kita, Kenji Yokota, Chihiro Kageyama, Susumu Take, Kazuyoshi Goto, Yoshiro Kawahara, Osamu Matsushita, Hiroyuki Okada

Research output: Contribution to journalArticle

Abstract

Helicobacter pylori infections cause gastritis and affect systemic immune responses; however, no direct association between immune cells and stomach bacteria has yet been reported. The present study investigated DEC205-mediated phagocytosis of H. pylori and the role of DEC205-positive macrophages in the human gastric mucosa. DEC205 mediated phagocytosis of H. pylori was detected immunocytochemically in PMAstimulated macrophages differentiated from NOMO1 cells. Expression of DEC205 mRNA in peripheral blood mononuclear cells (PBMCs) from H. pylori-infected patients was analyzed following stimulation with H. pylori cell lysate. We found that anti-DEC205 antibodies inhibited phagocytosis of H. pylori. The number of cells double-positive for DEC205 and CD14 in human gastric mucosa was higher in H. pylori-infected patients. DEC205-positive macrophages invaded the extracellular space between epithelial cells within gastric pits. In addition, DEC205 mRNA expression was upregulated in human PBMCs stimulated with H. pylori lysate. These findings suggest DEC205-expressing macrophages are important for recognition of H. pylori in human gastric mucosa, which affects systemic immunity.

Original languageEnglish
Pages (from-to)15828-15835
Number of pages8
JournalOncotarget
Volume9
Issue number22
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Helicobacter Infections
Helicobacter pylori
Gastric Mucosa
Macrophages
Phagocytosis
Blood Cells
Stomach
Messenger RNA
Extracellular Space
Gastritis
Anti-Idiotypic Antibodies
Immunity
Cell Count
Epithelial Cells
Bacteria

Keywords

  • CD14
  • DEC205
  • Helicobacter pylori
  • Immunology
  • Macrophage

ASJC Scopus subject areas

  • Oncology

Cite this

DEC205 mediates local and systemic immune responses to Helicobacter pylori infection in humans. / Kita, Masahide; Yokota, Kenji; Kageyama, Chihiro; Take, Susumu; Goto, Kazuyoshi; Kawahara, Yoshiro; Matsushita, Osamu; Okada, Hiroyuki.

In: Oncotarget, Vol. 9, No. 22, 01.01.2018, p. 15828-15835.

Research output: Contribution to journalArticle

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