De novo transcriptome analysis reveals an unperturbed transcriptome under high cadmium conditions in the Cd-hypertolerant fern Athyrium yokoscense

Yuko Ukai, Komaki Inoue, Manaka Kamada, Hiroshi Teramura, Shunsuke Yanagisawa, Kazuyoshi Kitazaki, Kazuhiro Shoji, Fumiyuki Goto, Keiichi Mochida, Toshihiro Yoshihara, Hiroaki Shimada

Research output: Contribution to journalArticle

Abstract

Athyrium yokoscense shows strong tolerance to cadmium exposure, even at levels that are many times greater than the toxic levels in ordinary plants. To determine the mechanism of Cd tolerance in A. yokoscense, we grew these plants under high Cd conditions and observed the tissue-specific accumulation of Cd and generation of reactive oxygen species, which is one of the major physiological responses to Cd stress. Fuchsin staining indicated the existence of a casparian strip in A. yokoscense roots, which may participate in Cd hypertolerance in A. yokoscense. Moreover, we performed RNA-seq of RNA samples from A. yokoscense plants treated with or without Cd exposure and obtained comprehensive RNA sequences as well as the Cd-responsive expression patterns of individual genes. Through de novo transcriptome assembly and gene expression analysis, we found that A. yokoscense showed normal features with no significant change in the expression levels of any transporter genes, even under high Cd exposure conditions. Our results demonstrate that A. yokoscense has an unusual mechanism that allows the invading Cd to partition into the distal roots, thus avoiding translocation of Cd into the xylem.

Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalGenes & genetic systems
Volume95
Issue number2
DOIs
Publication statusPublished - Jul 8 2020

Keywords

  • Athyrium yokoscense
  • cadmium tolerance
  • casparian strip
  • fern genome
  • transcriptome analysis
  • transporter gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Ukai, Y., Inoue, K., Kamada, M., Teramura, H., Yanagisawa, S., Kitazaki, K., Shoji, K., Goto, F., Mochida, K., Yoshihara, T., & Shimada, H. (2020). De novo transcriptome analysis reveals an unperturbed transcriptome under high cadmium conditions in the Cd-hypertolerant fern Athyrium yokoscense. Genes & genetic systems, 95(2), 65-74. https://doi.org/10.1266/ggs.19-00056