De-coupling of blood flow and metabolism in the rat brain induced by glutamate

Shinichiro Hirose, Sotaro Momosaki, Kazunari Sasaki, Rie Hosoi, Koji Abe, Antony Gee, Osamu Inoue

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: Glutamate plays an essential role in neuronal cell death in many neurological disorders. In this study, we examined both glucose metabolism and cerebral blood flow in the same rat following infusion of glutamate or ibotenic acid using the dual-tracer technique. The effects of MK-801, an NMDA receptor antagonist, and NBQX, an AMPA-kainate receptor antagonist, on the changes in the glucose metabolism and cerebral blood flow induced by glutamate were also examined. Methods: The rats were microinjected with glutamate (1 μmol/μl, 2 μl) or ibotenic acid (10 μg/μl, 1 μl) into the right striatum, and dual-tracer autoradiograms of [18F]FDG and [14C]IMP were obtained. MK-801 and NBQX were injected intravenously about 45 and 30 min, respectively, after the infusion of glutamate. Results: De-coupling of blood flow and metabolism was noted in the glutamate-infused hemisphere (as assessed by no alteration of [18F]FDG uptake and significant decrease of [14C]IMP uptake). Pretreatments with MK-801, NBQX, or combined use of MK-801 and NBQX did not affect the de-coupling of the blood flow and metabolism induced by glutamate. A histochemical study revealed that about 20% neuronal cell death had occurred in the striatum at 105 min after the infusion of glutamate. In addition, a significant increase of the [18F]FDG uptake and decrease of [14C]IMP uptake were also seen in the rat brain infused with ibotenic acid. Conclusion: These results indicate that glutamate and ibotenic acid caused a significant de-coupling of blood flow and glucose metabolism in the intact rat brain during the early phase of neurodegeneration. It is necessary to evaluate the relation between metabotropic glutamate receptors and de-coupling of blood flow and metabolism.

Original languageEnglish
Pages (from-to)293-300
Number of pages8
JournalAnnals of Nuclear Medicine
Volume23
Issue number3
DOIs
Publication statusPublished - May 2009
Externally publishedYes

Fingerprint

Glutamic Acid
Ibotenic Acid
Brain
Dizocilpine Maleate
Inosine Monophosphate
Cerebrovascular Circulation
Fluorodeoxyglucose F18
Cell Death
Kainic Acid Receptors
Glucose
Metabotropic Glutamate Receptors
AMPA Receptors
Nervous System Diseases
N-Methyl-D-Aspartate Receptors
Blood Glucose
2,3-dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline

Keywords

  • [C]IMP
  • [F]FDG
  • Brain
  • Glutamate
  • Rat

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

De-coupling of blood flow and metabolism in the rat brain induced by glutamate. / Hirose, Shinichiro; Momosaki, Sotaro; Sasaki, Kazunari; Hosoi, Rie; Abe, Koji; Gee, Antony; Inoue, Osamu.

In: Annals of Nuclear Medicine, Vol. 23, No. 3, 05.2009, p. 293-300.

Research output: Contribution to journalArticle

Hirose, S, Momosaki, S, Sasaki, K, Hosoi, R, Abe, K, Gee, A & Inoue, O 2009, 'De-coupling of blood flow and metabolism in the rat brain induced by glutamate', Annals of Nuclear Medicine, vol. 23, no. 3, pp. 293-300. https://doi.org/10.1007/s12149-009-0247-3
Hirose, Shinichiro ; Momosaki, Sotaro ; Sasaki, Kazunari ; Hosoi, Rie ; Abe, Koji ; Gee, Antony ; Inoue, Osamu. / De-coupling of blood flow and metabolism in the rat brain induced by glutamate. In: Annals of Nuclear Medicine. 2009 ; Vol. 23, No. 3. pp. 293-300.
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AU - Hirose, Shinichiro

AU - Momosaki, Sotaro

AU - Sasaki, Kazunari

AU - Hosoi, Rie

AU - Abe, Koji

AU - Gee, Antony

AU - Inoue, Osamu

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