DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication

Yasuo Ariumi, Misao Kuroki, Ken Ichi Abe, Hiromichi Dansako, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

Original languageEnglish
Pages (from-to)13922-13926
Number of pages5
JournalJournal of Virology
Volume81
Issue number24
DOIs
Publication statusPublished - Dec 2007

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DEAD-box RNA Helicases
Hepatitis C virus
Virus Replication
Hepacivirus
RNA
Genotype
Lentivirus
Replicon
replicon
Small Interfering RNA
genotype
virus replication
small interfering RNA
Cell Culture Techniques
Genome
cell culture
DEAD-box RNA helicases
RNA replication
cells
genome

ASJC Scopus subject areas

  • Immunology

Cite this

DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication. / Ariumi, Yasuo; Kuroki, Misao; Abe, Ken Ichi; Dansako, Hiromichi; Ikeda, Masanori; Wakita, Takaji; Kato, Nobuyuki.

In: Journal of Virology, Vol. 81, No. 24, 12.2007, p. 13922-13926.

Research output: Contribution to journalArticle

Ariumi, Yasuo ; Kuroki, Misao ; Abe, Ken Ichi ; Dansako, Hiromichi ; Ikeda, Masanori ; Wakita, Takaji ; Kato, Nobuyuki. / DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication. In: Journal of Virology. 2007 ; Vol. 81, No. 24. pp. 13922-13926.
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