DDX3 DEAD-box RNA helicase is required for hepatitis C virus RNA replication

Yasuo Ariumi, Misao Kuroki, Ken Ichi Abe, Hiromichi Dansako, Masanori Ikeda, Takaji Wakita, Nobuyuki Kato

Research output: Contribution to journalArticlepeer-review

185 Citations (Scopus)

Abstract

DDX3, a DEAD-box RNA helicase, binds to the hepatitis C virus (HCV) core protein. However, the role(s) of DDX3 in HCV replication is still not understood. Here we demonstrate that the accumulation of both genome-length HCV RNA (HCV-O, genotype 1b) and its replicon RNA were significantly suppressed in HuH-7-derived cells expressing short hairpin RNA targeted to DDX3 by lentivirus vector transduction. As well, RNA replication of JFH1 (genotype 2a) and release of the core into the culture supernatants were suppressed in DDX3 knockdown cells after inoculation of the cell culture-generated HCVcc. Thus, DDX3 is required for HCV RNA replication.

Original languageEnglish
Pages (from-to)13922-13926
Number of pages5
JournalJournal of Virology
Volume81
Issue number24
DOIs
Publication statusPublished - Dec 2007

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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