TY - JOUR
T1 - Dantrolene sodium reverses the increase in cAMP response element and TPA responsive element DNA-binding activity in the rabbit brain following haloperidol administration and heat stress
AU - Tanii, Hisashi
AU - Taniguchi, Norio
AU - Tsujio, Ichiro
AU - Asanuma, Masato
AU - Iwata, Emi
AU - Kudo, Takashi
AU - Ogawa, Norio
AU - Takeda, Masatoshi
PY - 1997
Y1 - 1997
N2 - Using electrophoretic mobility-shift assay (EMSA), were examined DNA- binding activity of cAMP response element (CRE), onto its responsive element CRE, as well as TPA consecutive element (TRE) in the medial hypothalamus and striatum of the experimental rabbits administered with haloperidol under heat stress exposure and studied the effects of dantrolene sodium to the transcriptional factor. In EMSA with nuclear extracts from the rabbit brain, the DNA-binding activities of CRE and TRE in medial hypothalamus and striatum increased following haloperidol and heat stress. These increases were maintained by coadministration with atropine. The treatment with dantrolene sodium markedly reversed such increases. The alterations of activities of these transcriptional factors may reflect the therapeutic effect of dantrolene sodium.
AB - Using electrophoretic mobility-shift assay (EMSA), were examined DNA- binding activity of cAMP response element (CRE), onto its responsive element CRE, as well as TPA consecutive element (TRE) in the medial hypothalamus and striatum of the experimental rabbits administered with haloperidol under heat stress exposure and studied the effects of dantrolene sodium to the transcriptional factor. In EMSA with nuclear extracts from the rabbit brain, the DNA-binding activities of CRE and TRE in medial hypothalamus and striatum increased following haloperidol and heat stress. These increases were maintained by coadministration with atropine. The treatment with dantrolene sodium markedly reversed such increases. The alterations of activities of these transcriptional factors may reflect the therapeutic effect of dantrolene sodium.
KW - Dantrolene sodium
KW - Electrophoretic mobility-shift assay
KW - Medial hypothalamus
KW - Striatum
KW - TPA responsive element
KW - cAMP response element
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U2 - 10.1111/j.1440-1819.1997.tb02610.x
DO - 10.1111/j.1440-1819.1997.tb02610.x
M3 - Article
C2 - 9472129
AN - SCOPUS:0031450415
VL - 51
SP - 415
EP - 420
JO - Psychiatry and Clinical Neurosciences
JF - Psychiatry and Clinical Neurosciences
SN - 1323-1316
IS - 6
ER -
