D14, a strigolactone-Insensitive mutant of rice, shows an accelerated outgrowth of tillers

Tomotsugu Arite, Mikihisa Umehara, Shinji Ishikawa, Atsushi Hanada, Masahiko Maekawa, Shinjiro Yamaguchi, Junko Kyozuka

Research output: Contribution to journalArticle

322 Citations (Scopus)

Abstract

Recent studies using highly branched mutants of pea, Arabidopsis and rice have demonstrated that strigolactones, a group of terpenoid lactones, act as a new hormone class, or its biosynthetic precursors, in inhibiting shoot branching. Here, we provide evidence that DWARF14 (D14) inhibits rice tillering and may act as a new compo-nent of the strigolactone-dependent branching inhibition pathway. The d14 mutant exhibits increased shoot branch-ing with reduced plant height like the previously characterized strigolactone-deficient and -insensitive mutants d10 and d3, respectively. The d10-1 d14-1 double mutant is phenotypically indistinguishable from the d10-1 and d14-1 single mutants, consistent with the idea that D10 and D14 function in the same pathway. However, unlike with d10, the d14 branching phenotype could not be rescued by exogenous strigolactones. In addition, the d14 mutant contained a higher level of 2′-epi-5-deoxystrigol than the wild type. Positional cloning revealed that D14 encodes a protein of the α/β-fold hydrolase superfamily, some members of which play a role in metabolism or signaling of plant hormones. We propose that D14 functions downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form.

Original languageEnglish
Pages (from-to)1416-1424
Number of pages9
JournalPlant and Cell Physiology
Volume50
Issue number8
DOIs
Publication statusPublished - Aug 2009

Fingerprint

tillers
Hormones
rice
mutants
Plant Growth Regulators
Terpenes
Peas
Hydrolases
Lactones
Arabidopsis
branching
Organism Cloning
Phenotype
Enzymes
hormones
Proteins
shoots
tillering
hydrolases
lactones

Keywords

  • DWARF 14
  • Hormone signaling
  • Rice
  • Shoot branching
  • Strigolactone

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Arite, T., Umehara, M., Ishikawa, S., Hanada, A., Maekawa, M., Yamaguchi, S., & Kyozuka, J. (2009). D14, a strigolactone-Insensitive mutant of rice, shows an accelerated outgrowth of tillers. Plant and Cell Physiology, 50(8), 1416-1424. https://doi.org/10.1093/pcp/pcp091

D14, a strigolactone-Insensitive mutant of rice, shows an accelerated outgrowth of tillers. / Arite, Tomotsugu; Umehara, Mikihisa; Ishikawa, Shinji; Hanada, Atsushi; Maekawa, Masahiko; Yamaguchi, Shinjiro; Kyozuka, Junko.

In: Plant and Cell Physiology, Vol. 50, No. 8, 08.2009, p. 1416-1424.

Research output: Contribution to journalArticle

Arite, T, Umehara, M, Ishikawa, S, Hanada, A, Maekawa, M, Yamaguchi, S & Kyozuka, J 2009, 'D14, a strigolactone-Insensitive mutant of rice, shows an accelerated outgrowth of tillers', Plant and Cell Physiology, vol. 50, no. 8, pp. 1416-1424. https://doi.org/10.1093/pcp/pcp091
Arite T, Umehara M, Ishikawa S, Hanada A, Maekawa M, Yamaguchi S et al. D14, a strigolactone-Insensitive mutant of rice, shows an accelerated outgrowth of tillers. Plant and Cell Physiology. 2009 Aug;50(8):1416-1424. https://doi.org/10.1093/pcp/pcp091
Arite, Tomotsugu ; Umehara, Mikihisa ; Ishikawa, Shinji ; Hanada, Atsushi ; Maekawa, Masahiko ; Yamaguchi, Shinjiro ; Kyozuka, Junko. / D14, a strigolactone-Insensitive mutant of rice, shows an accelerated outgrowth of tillers. In: Plant and Cell Physiology. 2009 ; Vol. 50, No. 8. pp. 1416-1424.
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