D1 fragmentation in photosystem II repair caused by photo-damage of a two-step model

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Light energy drives photosynthesis, but it simultaneously inactivates photosynthetic mechanisms. A major target site of photo-damage is photosystem II (PSII). It further targets one reaction center protein, D1, which is maintained efficiently by the PSII repair cycle. Two proteases, FtsH and Deg, are known to contribute to this process, respectively, by efficient degradation of photo-damaged D1 protein processively and endoproteolytically. This study tested whether the D1 cleavage accomplished by these proteases is affected by different monochromic lights such as blue and red light-emitting-diode light sources, remaining mindful that the use of these lights distinguishes the current models for photoinhibition: the excess-energy model and the two-step model. It is noteworthy that in the two-step model, primary damage results from the absorption of light energy in the Mn-cluster, which can be enhanced by a blue rather than a red light source. Results showed that blue and red lights affect D1 degradation differently. One prominent finding was that D1 fragmentation that is specifically generated by luminal Deg proteases was enhanced by blue light but not by red light in the mutant lacking FtsH2. Although circumstantial, this evidence supports a two-step model of PSII photo-damage. We infer that enhanced D1 fragmentation by luminal Deg proteases is a response to primary damage at the Mn-cluster.

Original languageEnglish
Pages (from-to)409-416
Number of pages8
JournalPhotosynthesis research
Volume126
Issue number2-3
DOIs
Publication statusPublished - Dec 1 2015

Keywords

  • Chloroplast protease
  • D1 degradation
  • Deg
  • FtsH
  • PSII repair
  • Photoinhibition

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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