D-Aspartate modulates melatonin synthesis in rat pinealocytes

Shougo Ishio; Hiroshi Yamada; Mitsuko Hayashi; Shouki Yatsushiro; Takato Noumi; Akihito Yamaguchi; Yoshinori Moriyama

doi:10.1016/S0304-3940(98)00414-5

D-Aspartate modulates melatonin synthesis in rat pinealocytes

Shougo Ishio, Hiroshi Yamada, Mitsuko Hayashi, Shouki Yatsushiro, Takato Noumi, Akihito Yamaguchi, Yoshinori Moriyama

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

77 Citations (Scopus)

Abstract

It has been known that pinealocytes contain the highest level of D- aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 μM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D- aspartate acts as a negative regulator for melatonin synthesis in the pineal gland.

Original language	English
Pages (from-to)	143-146
Number of pages	4
Journal	Neuroscience Letters
Volume	249
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(98)00414-5
Publication status	Published - Jun 19 1998

Keywords

Cyclic AMP
D-Aspartate
L-Glutamate
Melatonin
Pinealocyte
Regulation
Serotonin N- acetyltransferase

ASJC Scopus subject areas

Neuroscience(all)

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title = "D-Aspartate modulates melatonin synthesis in rat pinealocytes",

abstract = "It has been known that pinealocytes contain the highest level of D- aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 μM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D- aspartate acts as a negative regulator for melatonin synthesis in the pineal gland.",

keywords = "Cyclic AMP, D-Aspartate, L-Glutamate, Melatonin, Pinealocyte, Regulation, Serotonin N- acetyltransferase",

author = "Shougo Ishio and Hiroshi Yamada and Mitsuko Hayashi and Shouki Yatsushiro and Takato Noumi and Akihito Yamaguchi and Yoshinori Moriyama",

note = "Funding Information: H.Y. and S.Y. were supported by Research Fellowship of the Japan Society from Promotion of Science for Young Scientists. This work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan, the Terumo Science Foundation, the Chiba-Geigy Foundation (Japan) for the Promotion of Science, and the Salt Science Research Foundation.",

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AU - Ishio, Shougo

AU - Yamada, Hiroshi

AU - Hayashi, Mitsuko

AU - Yatsushiro, Shouki

AU - Noumi, Takato

AU - Yamaguchi, Akihito

AU - Moriyama, Yoshinori

N1 - Funding Information: H.Y. and S.Y. were supported by Research Fellowship of the Japan Society from Promotion of Science for Young Scientists. This work was supported in part by grants from the Ministry of Education, Science, Sports and Culture of Japan, the Terumo Science Foundation, the Chiba-Geigy Foundation (Japan) for the Promotion of Science, and the Salt Science Research Foundation.

PY - 1998/6/19

Y1 - 1998/6/19

N2 - It has been known that pinealocytes contain the highest level of D- aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 μM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D- aspartate acts as a negative regulator for melatonin synthesis in the pineal gland.

AB - It has been known that pinealocytes contain the highest level of D- aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 μM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D- aspartate acts as a negative regulator for melatonin synthesis in the pineal gland.

KW - Cyclic AMP

KW - D-Aspartate

KW - L-Glutamate

KW - Melatonin

KW - Pinealocyte

KW - Regulation

KW - Serotonin N- acetyltransferase

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D-Aspartate modulates melatonin synthesis in rat pinealocytes

Abstract

Keywords

ASJC Scopus subject areas

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