D-Aspartate modulates melatonin synthesis in rat pinealocytes

Shougo Ishio; Hiroshi Yamada; Mitsuko Hayashi; Shouki Yatsushiro; Takato Noumi; Akihito Yamaguchi; Yoshinori Moriyama

doi:10.1016/S0304-3940(98)00414-5

D-Aspartate modulates melatonin synthesis in rat pinealocytes

Shougo Ishio, Hiroshi Yamada, Mitsuko Hayashi, Shouki Yatsushiro, Takato Noumi, Akihito Yamaguchi, Yoshinori Moriyama

Faculty of Pharmaceutical Sciences

Research output: Contribution to journal › Article

75 Citations (Scopus)

Abstract

It has been known that pinealocytes contain the highest level of D- aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 μM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D- aspartate acts as a negative regulator for melatonin synthesis in the pineal gland.

Original language	English
Pages (from-to)	143-146
Number of pages	4
Journal	Neuroscience Letters
Volume	249
Issue number	2-3
DOIs	https://doi.org/10.1016/S0304-3940(98)00414-5
Publication status	Published - Jun 19 1998

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Keywords

Cyclic AMP
D-Aspartate
L-Glutamate
Melatonin
Pinealocyte
Regulation
Serotonin N- acetyltransferase

ASJC Scopus subject areas

Neuroscience(all)

Cite this

Ishio, S., Yamada, H., Hayashi, M., Yatsushiro, S., Noumi, T., Yamaguchi, A., & Moriyama, Y. (1998). D-Aspartate modulates melatonin synthesis in rat pinealocytes. Neuroscience Letters, 249(2-3), 143-146. https://doi.org/10.1016/S0304-3940(98)00414-5

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abstract = "It has been known that pinealocytes contain the highest level of D- aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 μM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D- aspartate acts as a negative regulator for melatonin synthesis in the pineal gland.",

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Access to Document

10.1016/S0304-3940(98)00414-5