D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy

Masanori Mizote, Kazuyuki Hirooka, Kouki Fukuda, Takehiro Nakamura, Toshifumi Itano, Fumio Shiraga

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: To investigate the protective effects of d-allose, a rare sugar, on pressure-induced ischemia during vitrectomy in the rabbit eye. Methods: The rabbits underwent pars plana vitrectomy, and continuous intraocular irrigation at a perfusion pressure of 140 mmHg was performed for 45 min. Intraocular pressure was regulated by adjusting the height of a bottle of balanced saline solution containing d-allose. Morphometric studies were performed to study the effects of d-allose on the histological changes induced by ischemia in the rabbit retina. Electroretinograms (ERGs) were taken before and 1 and 7 days after vitrectomy. Nitroblue tetrazolium was used as an index of superoxide anion (O 2 •- ) generation. Data were analyzed by use of the unpaired Student's t test. Results: Seven days after ischemia, significant reductions in both number of ganglion cells and the thickness of the inner plexiform layer were observed. d-Allose significantly inhibited ischemic injury of the inner retina (P < 0.05). On postoperative day 7, amplitudes of ERG b-waves were significantly lower in the control group than in the d-allose group (P < 0.05). d-Allose suppressed the production of O2 -. Conclusions: Intraocular irrigation with d-allose during vitrectomy may protect the retina against ischemia-induced damage.

Original languageEnglish
Pages (from-to)294-300
Number of pages7
JournalJapanese Journal of Ophthalmology
Volume55
Issue number3
DOIs
Publication statusPublished - May 1 2011
Externally publishedYes

Keywords

  • Neuroprotection
  • Retinal ischemia
  • Superoxide anion
  • Vitrectomy
  • d-Allose

ASJC Scopus subject areas

  • Ophthalmology

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  • Cite this

    Mizote, M., Hirooka, K., Fukuda, K., Nakamura, T., Itano, T., & Shiraga, F. (2011). D-Allose as ischemic retina injury inhibitor during rabbit vitrectomy. Japanese Journal of Ophthalmology, 55(3), 294-300. https://doi.org/10.1007/s10384-011-0012-8