Cytotoxicity of benzyl isothiocyanate in normal renal proximal tubular cells and its modulation by glutathione

Naomi Abe, Masashi Okuhira, Chiharu Tsutsui, Yoshiyuki Murata, Yoshimasa Nakamura

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In the present study, we examined the toxicity of benzyl ITC (BITC) and its urinary mercapturic acid metabolite (BITC-NAC), using a normal renal proximal tubular cell line, pig LLC-PK1. BITC increased cell death with an IC 50value of about 7 μM, whereas the cytotoxic effect of BITC-NAC was five times weaker than that of BITC. We observed a significant necrosis of the compounds on LLC-PK1 cells with oxidative stress. In the presence of 5 mM glutathione (GSH), comparable to physiological levels, the cytotoxicity of BITC-NAC as well as BITC was significantly reduced. Furthermore, the increase in intracellular GSH levels by pretreatment with NAC before the BITC treatment resulted in inhibition of the BITC-induced necrotic events as well as intracellular oxidative stress. These results suggest that GSH is a determinant of cellular resistance against the BITC-mediated and oxidative stress-dependent cytotoxicity in renal proximal tubular cells.

Original languageEnglish
Pages (from-to)1887-1892
Number of pages6
JournalJournal of agricultural and food chemistry
Volume60
Issue number7
DOIs
Publication statusPublished - Feb 22 2012

Keywords

  • ATP
  • LLC-PK1 cell
  • benzyl isothiocyanate
  • necrosis
  • reactive oxygen species

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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