Cytotoxicity and cancer detection ability of the luminescent nanoporous silica spheres immobilized with folic acid derivative

Motohiro Tagaya, Nobutaka Hanagata, Toshiyuki Ikoma, Takaomi Kobayashi, Kota Shiba, Tomohiko Yoshioka, Junzo Tnaka

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

Luminescent europium(III)-doped nanoporous silica spheres (Eu:NPS) were synthesized, and folic acid derivative (FA: folate N-hydroxysuccinimidyl ester) as targeting ligand for cancer cells was immobilized on the sphere through a mediation of 3-aminopropyltriethoxysilane (APTES). The ordered nanopores were preserved with the immobilization. The surface area decreased only with the APTES immobilization, suggesting that the FA was immobilized only on the outer surface of the nanopores. The photoluminescence of the spheres showed the characteristic peak due to interactions between the FA and Eu3+, and further the luminescence can be clearly detected by a fluorescent microscopy. The spheres were highly dispersed stability in cell culture medium to exhibit the nontoxic in the proliferation stage of Hela cancer cells and NIH3T3 fibroblasts, and specifically bindto the Hela cells. The binding and uptake spheres showed the intense luminescence. Thus, the luminescent FA-immobilized Eu:NPS spheres will exhibit the targeting and imaging abilities for cancer cells.

Original languageEnglish
Title of host publicationBioceramics 24
Pages630-635
Number of pages6
Edition1
DOIs
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012 - Fukuoka, Japan
Duration: Oct 21 2012Oct 24 2012

Publication series

NameKey Engineering Materials
Number1
Volume529-530
ISSN (Print)1013-9826

Other

Other24th Symposium and Annual Meeting of International Society for Ceramics in Medicine, ISCM 2012
CountryJapan
CityFukuoka
Period10/21/1210/24/12

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Keywords

  • Bio-imaging
  • Cancer cells
  • Nanoparticles
  • Nanoporous silicas
  • Photoluminescence

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Tagaya, M., Hanagata, N., Ikoma, T., Kobayashi, T., Shiba, K., Yoshioka, T., & Tnaka, J. (2013). Cytotoxicity and cancer detection ability of the luminescent nanoporous silica spheres immobilized with folic acid derivative. In Bioceramics 24 (1 ed., pp. 630-635). (Key Engineering Materials; Vol. 529-530, No. 1). https://doi.org/10.4028/www.scientific.net/KEM.529-530.630