Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines - A possible mechanism

H. Sakagami, Y. Jiang, K. Kusama, T. Atsumi, T. Ueha, M. Toguchi, I. Iwakura, K. Satoh, H. Ito, Tsutomu Hatano, T. Yoshida

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Hydrolyzable tannins showed higher cytotoxic activity against human oral squamous cell carcinoma and salivary gland tumor cell lines than against normal human gingival fibroblasts, whereas gallic acid, a component unit of tannins, showed much weaker selective cytotoxicity. The cytotoxic activity of dimeric compounds was generally higher than that of monomeric compounds. Macrocyclic ellagitannin oligomers, such as oenothein B, woodfordin C and woodfordin D showed the greatest cytotoxic activity, and their activity (per given number of molecules) was one order higher than those of gallic acid and epigallocatechin gallate, a major component of green tea. These compounds induced apoptotic cell death characterized by DNA fragmentation (as demonstrated by the TUNEL method) and cleavage of cytokeratin 18 by activated caspase(s) (as demonstrated by M30 monoclonal antibody). ESR spectroscopy revealed that these macrocyclic compounds at higher concentrations produced their own radicals and significantly enhanced the radical intensity of sodium ascorbate, possibly by their prooxidant actions. Catalase failed to eliminate their apoptosis-inducing activity, reducing the possibility of the involvement of hydrogen peroxide production in the extracellular fraction. These observations suggested that the antitumor activity of macrocyclic ellagitannin oligomers reported previously might be explained by their apoptosis-inducing activity.

Original languageEnglish
Pages (from-to)39-47
Number of pages9
JournalPhytomedicine
Volume7
Issue number1
Publication statusPublished - Mar 2000

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Hydrolyzable Tannins
Gallic Acid
Tumor Cell Line
gallic acid
Oligomers
tannins
Tumors
mouth
Macrocyclic Compounds
apoptosis
Cells
cell lines
Apoptosis
Keratin-18
Glandular and Epithelial Neoplasms
epigallocatechin
Tannins
caspases
DNA fragmentation
In Situ Nick-End Labeling

Keywords

  • Apoptosis
  • Caspase
  • DNA fragmentation
  • Macrocyclic ellagitannins
  • Oral tumor cells

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Sakagami, H., Jiang, Y., Kusama, K., Atsumi, T., Ueha, T., Toguchi, M., ... Yoshida, T. (2000). Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines - A possible mechanism. Phytomedicine, 7(1), 39-47.

Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines - A possible mechanism. / Sakagami, H.; Jiang, Y.; Kusama, K.; Atsumi, T.; Ueha, T.; Toguchi, M.; Iwakura, I.; Satoh, K.; Ito, H.; Hatano, Tsutomu; Yoshida, T.

In: Phytomedicine, Vol. 7, No. 1, 03.2000, p. 39-47.

Research output: Contribution to journalArticle

Sakagami, H, Jiang, Y, Kusama, K, Atsumi, T, Ueha, T, Toguchi, M, Iwakura, I, Satoh, K, Ito, H, Hatano, T & Yoshida, T 2000, 'Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines - A possible mechanism', Phytomedicine, vol. 7, no. 1, pp. 39-47.
Sakagami H, Jiang Y, Kusama K, Atsumi T, Ueha T, Toguchi M et al. Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines - A possible mechanism. Phytomedicine. 2000 Mar;7(1):39-47.
Sakagami, H. ; Jiang, Y. ; Kusama, K. ; Atsumi, T. ; Ueha, T. ; Toguchi, M. ; Iwakura, I. ; Satoh, K. ; Ito, H. ; Hatano, Tsutomu ; Yoshida, T. / Cytotoxic activity of hydrolyzable tannins against human oral tumor cell lines - A possible mechanism. In: Phytomedicine. 2000 ; Vol. 7, No. 1. pp. 39-47.
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