TY - JOUR
T1 - Cytosolic alkalization and cytosolic calcium oscillation in Arabidopsis guard cells Response to ABA and MeJA
AU - Islam, Mohammad Mahbub
AU - Hossain, Mohammad Anowar
AU - Jannat, Rayhanur
AU - Munemasa, Shintaro
AU - Nakamura, Yoshimasa
AU - Mori, Izumi C.
AU - Murata, Yoshiyuki
N1 - Funding Information:
This research was funded in part by a Grant-in-Aid for Young Scientists and Grants for Scientific Research on Priority Areas from the Ministry of Education, Culture, Sports, Science and Technology of Japan.
PY - 2010/10
Y1 - 2010/10
N2 - Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.
AB - Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.
KW - Abscisic acid
KW - Arabidopsis thaliana
KW - Cytosolic calcium
KW - Cytosolic pH
KW - Guard cells
KW - Methyl jasmonate
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U2 - 10.1093/pcp/pcq131
DO - 10.1093/pcp/pcq131
M3 - Article
C2 - 20739306
AN - SCOPUS:77957945502
VL - 51
SP - 1721
EP - 1730
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 10
ER -