Cytosolic alkalization and cytosolic calcium oscillation in Arabidopsis guard cells Response to ABA and MeJA

Mohammad Mahbub Islam, Mohammad Anowar Hossain, Rayhanur Jannat, Shintaro Munemasa, Yoshimasa Nakamura, Izumi Mori, Yoshiyuki Murata

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)1721-1730
Number of pages10
JournalPlant and Cell Physiology
Volume51
Issue number10
DOIs
Publication statusPublished - Oct 2010

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Keywords

  • Abscisic acid
  • Arabidopsis thaliana
  • Cytosolic calcium
  • Cytosolic pH
  • Guard cells
  • Methyl jasmonate

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

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