Abstract
Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1721-1730 |
| Number of pages | 10 |
| Journal | Plant and Cell Physiology |
| Volume | 51 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 2010 |
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Keywords
- Abscisic acid
- Arabidopsis thaliana
- Cytosolic calcium
- Cytosolic pH
- Guard cells
- Methyl jasmonate
ASJC Scopus subject areas
- Plant Science
- Physiology
- Cell Biology
Cite this
Cytosolic alkalization and cytosolic calcium oscillation in Arabidopsis guard cells Response to ABA and MeJA. / Islam, Mohammad Mahbub; Hossain, Mohammad Anowar; Jannat, Rayhanur; Munemasa, Shintaro; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.
In: Plant and Cell Physiology, Vol. 51, No. 10, 10.2010, p. 1721-1730.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytosolic alkalization and cytosolic calcium oscillation in Arabidopsis guard cells Response to ABA and MeJA
AU - Islam, Mohammad Mahbub
AU - Hossain, Mohammad Anowar
AU - Jannat, Rayhanur
AU - Munemasa, Shintaro
AU - Nakamura, Yoshimasa
AU - Mori, Izumi
AU - Murata, Yoshiyuki
PY - 2010/10
Y1 - 2010/10
N2 - Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.
AB - Abscisic acid (ABA)- and methyl jasmonate (MeJA)-induced stomatal closure are accompanied by cytosolic alkalization in guard cells. However, it remains to be clarified how the alkalization functions in not only ABA signaling but also MeJA. We investigated cytosolic alkalization in guard cells during ABA-, MeJA- and Ca2+-induced stomatal closure of wild type, abi1-1, abi2-1, ost1-2 and coi1 using a pH-sensitive fluorescent dye, BCECF-AM. ABA induced cytosolic alkalization in guard cells of wild-type and coi1 but not in ost1-2 guard cells whereas MeJA elicited cytosolic alkalization in wild-type and ost1-2 guard cells but not in coi1. Neither ABA nor MeJA induced cytosolic alkalization in abi1-1 and abi2-1 guard cells. Exogenous Ca2+ induced stomatal closure accompanied by cytosolic alkalization in guard cells of wild-type, abi1-1, abi2-1, ost1-2 and coi1 plants. An agent to acidify cytosol, butyrate, suppressed Ca2+-induced cytosolic alkalization and ABA-, MeJA- and Ca2+-induced cytosolic Ca2+ oscillation in wild-type guard cells to prevent stomatal closure. These results indicate that cytosolic alkalization and cytosolic Ca2+ oscillation coordinately function in ABA and MeJA signaling in Arabidopsis guard cells.
KW - Abscisic acid
KW - Arabidopsis thaliana
KW - Cytosolic calcium
KW - Cytosolic pH
KW - Guard cells
KW - Methyl jasmonate
UR - http://www.scopus.com/inward/record.url?scp=77957945502&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957945502&partnerID=8YFLogxK
U2 - 10.1093/pcp/pcq131
DO - 10.1093/pcp/pcq131
M3 - Article
C2 - 20739306
AN - SCOPUS:77957945502
VL - 51
SP - 1721
EP - 1730
JO - Plant and Cell Physiology
JF - Plant and Cell Physiology
SN - 0032-0781
IS - 10
ER -