Cytoskeleton and differentiation: effects of cytochalasin B and colchicine on expression of the differentiated phenotype of rabbit costal chondrocytes in culture

Masaharu Takigawa, Teruko Takano, Eiji Shirai, Fujio Suzuki

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cytochalasin B changed the shape of cultured rabbit costal chondrocytes from polygonal to nearly spherical and stimulated glycosaminoglycan synthesis, which is a differentiated phenotype of chondrocytes, whereas colchicine changed them from polygonal to flattened and inhibited glycosaminoglycan synthesis. These morphological changes occurred parallel with the changes in glycosaminoglycan synthesis. Induction of ornithine decarboxylase by parathyroid hormone, which is a good marker of differentiated chondrocytes, was markedly potentiated in the spherical cells which had been pretreated with cytochalasin B, whereas pretreatment with colchicine inhibited the induction of the enzyme. Both cytochalasin B and colchicine inhibited DNA synthesis. The inhibitions were observed after the appearance of changes in the morphology of the cells and glycosaminoglycan synthesis. These findings suggest that intactness of microtubules and disruption of microfilaments are involved in regulating the expression of the differentiated phenotype of chondrocytes in culture.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalCell Differentiation
Volume14
Issue number3
DOIs
Publication statusPublished - Aug 1984
Externally publishedYes

Keywords

  • cultured chondrocytes
  • cytoskeleton
  • differentiation
  • glycosaminoglycan synthesis
  • ornithine decarboxylase
  • parathyroid hormone

ASJC Scopus subject areas

  • Developmental Biology

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