Cytoplasmic delayed neuronal death in the myenteric plexus of the rat small intestine after ischemia

Da Xun Piao, Hong Chi Jiang, Motoyasu Kosaka, Takashi Shibata, Aiji Ohtsuka, Takuro Murakami

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The present study demonstrates light and electron microscopic changes in neurons in the myenteric plexus of the rat ileum following four-hour ischemia. Macroscopically, an intestinal constriction occurred at the damaged portion at three weeks after ischemia; the segment oral to the constriction markedly swelled at four weeks. In light microscopy, at three weeks after ischemia, the myenteric neurons appeared spongy or foamy, containing many vacuoles in their somatic cytoplasm. At four weeks, the neuronal cytoplasm and nerve fiber bundles had disintegrated to form vacant spaces in the myenteric plexus. The neuronal nucleus of the damaged plexus did not show positive nick-end labeling. In electron microscopy, neuronal cytoplasm revealed degenerative signs already at one week after ischemia: a distended endoplasmic reticulum and swollen mitochondria with fragmentary cristae. The nerve fibers also showed destruction of the mitochondria, and degenerative changes in the postsynaptic sites appeared earlier than the presynaptic terminals. The results suggest that intestinal ischemia causes delayed neuronal death, which differs from the apoptotic process previously demonstrated in the ischemia-damaged brain.

Original languageEnglish
Pages (from-to)383-392
Number of pages10
JournalArchives of Histology and Cytology
Volume62
Issue number4
Publication statusPublished - Oct 1999

Fingerprint

Myenteric Plexus
Small Intestine
Ischemia
Cytoplasm
Nerve Fibers
Constriction
Mitochondria
Neurons
Light
Presynaptic Terminals
Vacuoles
Brain Ischemia
Ileum
Endoplasmic Reticulum
Microscopy
Electron Microscopy
Electrons

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Cytoplasmic delayed neuronal death in the myenteric plexus of the rat small intestine after ischemia. / Piao, Da Xun; Jiang, Hong Chi; Kosaka, Motoyasu; Shibata, Takashi; Ohtsuka, Aiji; Murakami, Takuro.

In: Archives of Histology and Cytology, Vol. 62, No. 4, 10.1999, p. 383-392.

Research output: Contribution to journalArticle

Piao, Da Xun ; Jiang, Hong Chi ; Kosaka, Motoyasu ; Shibata, Takashi ; Ohtsuka, Aiji ; Murakami, Takuro. / Cytoplasmic delayed neuronal death in the myenteric plexus of the rat small intestine after ischemia. In: Archives of Histology and Cytology. 1999 ; Vol. 62, No. 4. pp. 383-392.
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