Cytoplasmic alkalization and cytoplasmic streaming induced by light and histidine in leaf cells of egeria densa: In vivo 31P-NMR study

Yoshito Tominaga, Kazuyuki Kuchitsu, Maki Katsuhara, Masashi Tazawa, Shigetoh Miyachi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rotational streaming of the cytoplasm including chloroplasts was induced by L-histidine, as well as by light, on the anticlinal face of leaf cells of Egeria densa. In the case of treatment with L-histidine some of the chloroplasts remained stationary on the periclinal face of cells after rotational cytoplasmic streaming was initiated. However, these chloroplasts were easily dislodged and translocated to the centrifugal end of the histidine-treated cells by application of a centrifugal force that barely affected the location of chloroplasts in cells incubated in the dark without L-histidine. This result indicates that the anchoring of chloroplasts was weakened by L-histidine. Thus only the release of chloroplasts from anchoring was not enough for initiation of their streaming.The cytoplasmic pH (pHc) and vacuolar pH (pHv) were noninvasively monitored by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Compared with the dark control value, both illumination and treatment with L-histidine increased the pHc by 0.3 units. In contrast, pHv changed only a little with both illumination and treatment with L-histidine. Release of chloroplasts from anchoring and initiation of cytoplasmic streaming are discussed in relation to the increase in pHc induced by both light and L-histidine.

Original languageEnglish
Pages (from-to)261-268
Number of pages8
JournalPlant and Cell Physiology
Volume32
Issue number2
Publication statusPublished - Mar 1991
Externally publishedYes

Fingerprint

Cytoplasmic Streaming
Egeria densa
cytoplasmic streaming
alkalinization
Nuclear Magnetic Resonance
Chloroplast
Streaming
histidine
Histidine
chloroplast
Chloroplasts
nuclear magnetic resonance
nuclear magnetic resonance spectroscopy
Leaves
Magnetic Resonance Spectroscopy
Lighting
Nuclear magnetic resonance
chloroplasts
Light
Cell

Keywords

  • 31P-NMR
  • Cytoplasmic streaming
  • Egeria densa
  • Histidine
  • Intracellular pH
  • Light

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Ecology
  • Cell Biology
  • Physiology
  • Plant Science

Cite this

Cytoplasmic alkalization and cytoplasmic streaming induced by light and histidine in leaf cells of egeria densa : In vivo 31P-NMR study. / Tominaga, Yoshito; Kuchitsu, Kazuyuki; Katsuhara, Maki; Tazawa, Masashi; Miyachi, Shigetoh.

In: Plant and Cell Physiology, Vol. 32, No. 2, 03.1991, p. 261-268.

Research output: Contribution to journalArticle

Tominaga, Yoshito ; Kuchitsu, Kazuyuki ; Katsuhara, Maki ; Tazawa, Masashi ; Miyachi, Shigetoh. / Cytoplasmic alkalization and cytoplasmic streaming induced by light and histidine in leaf cells of egeria densa : In vivo 31P-NMR study. In: Plant and Cell Physiology. 1991 ; Vol. 32, No. 2. pp. 261-268.
@article{951810f94474468c91ecaa90bb37ac6b,
title = "Cytoplasmic alkalization and cytoplasmic streaming induced by light and histidine in leaf cells of egeria densa: In vivo 31P-NMR study",
abstract = "Rotational streaming of the cytoplasm including chloroplasts was induced by L-histidine, as well as by light, on the anticlinal face of leaf cells of Egeria densa. In the case of treatment with L-histidine some of the chloroplasts remained stationary on the periclinal face of cells after rotational cytoplasmic streaming was initiated. However, these chloroplasts were easily dislodged and translocated to the centrifugal end of the histidine-treated cells by application of a centrifugal force that barely affected the location of chloroplasts in cells incubated in the dark without L-histidine. This result indicates that the anchoring of chloroplasts was weakened by L-histidine. Thus only the release of chloroplasts from anchoring was not enough for initiation of their streaming.The cytoplasmic pH (pHc) and vacuolar pH (pHv) were noninvasively monitored by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Compared with the dark control value, both illumination and treatment with L-histidine increased the pHc by 0.3 units. In contrast, pHv changed only a little with both illumination and treatment with L-histidine. Release of chloroplasts from anchoring and initiation of cytoplasmic streaming are discussed in relation to the increase in pHc induced by both light and L-histidine.",
keywords = "31P-NMR, Cytoplasmic streaming, Egeria densa, Histidine, Intracellular pH, Light",
author = "Yoshito Tominaga and Kazuyuki Kuchitsu and Maki Katsuhara and Masashi Tazawa and Shigetoh Miyachi",
year = "1991",
month = "3",
language = "English",
volume = "32",
pages = "261--268",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "2",

}

TY - JOUR

T1 - Cytoplasmic alkalization and cytoplasmic streaming induced by light and histidine in leaf cells of egeria densa

T2 - In vivo 31P-NMR study

AU - Tominaga, Yoshito

AU - Kuchitsu, Kazuyuki

AU - Katsuhara, Maki

AU - Tazawa, Masashi

AU - Miyachi, Shigetoh

PY - 1991/3

Y1 - 1991/3

N2 - Rotational streaming of the cytoplasm including chloroplasts was induced by L-histidine, as well as by light, on the anticlinal face of leaf cells of Egeria densa. In the case of treatment with L-histidine some of the chloroplasts remained stationary on the periclinal face of cells after rotational cytoplasmic streaming was initiated. However, these chloroplasts were easily dislodged and translocated to the centrifugal end of the histidine-treated cells by application of a centrifugal force that barely affected the location of chloroplasts in cells incubated in the dark without L-histidine. This result indicates that the anchoring of chloroplasts was weakened by L-histidine. Thus only the release of chloroplasts from anchoring was not enough for initiation of their streaming.The cytoplasmic pH (pHc) and vacuolar pH (pHv) were noninvasively monitored by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Compared with the dark control value, both illumination and treatment with L-histidine increased the pHc by 0.3 units. In contrast, pHv changed only a little with both illumination and treatment with L-histidine. Release of chloroplasts from anchoring and initiation of cytoplasmic streaming are discussed in relation to the increase in pHc induced by both light and L-histidine.

AB - Rotational streaming of the cytoplasm including chloroplasts was induced by L-histidine, as well as by light, on the anticlinal face of leaf cells of Egeria densa. In the case of treatment with L-histidine some of the chloroplasts remained stationary on the periclinal face of cells after rotational cytoplasmic streaming was initiated. However, these chloroplasts were easily dislodged and translocated to the centrifugal end of the histidine-treated cells by application of a centrifugal force that barely affected the location of chloroplasts in cells incubated in the dark without L-histidine. This result indicates that the anchoring of chloroplasts was weakened by L-histidine. Thus only the release of chloroplasts from anchoring was not enough for initiation of their streaming.The cytoplasmic pH (pHc) and vacuolar pH (pHv) were noninvasively monitored by in vivo 31P-nuclear magnetic resonance (NMR) spectroscopy. Compared with the dark control value, both illumination and treatment with L-histidine increased the pHc by 0.3 units. In contrast, pHv changed only a little with both illumination and treatment with L-histidine. Release of chloroplasts from anchoring and initiation of cytoplasmic streaming are discussed in relation to the increase in pHc induced by both light and L-histidine.

KW - 31P-NMR

KW - Cytoplasmic streaming

KW - Egeria densa

KW - Histidine

KW - Intracellular pH

KW - Light

UR - http://www.scopus.com/inward/record.url?scp=0008930013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0008930013&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0008930013

VL - 32

SP - 261

EP - 268

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 2

ER -