Cytological karyotyping of three Cochliobolus spp. by the Germ tube burst method

D. Tsuchiya, M. Taga

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Cytological karyotypes with mitotic metaphase chromosomes were analyzed for Cochliobolus heterostrophus, C. carbonum, and C. sativus by the germ tube burst method (GTBM). Prior to karyotyping, procedures of GTBM suitable to Cochliobolus were established by examining several crucial conditions such as incubation period of conidia. The estimated chromosome numbers of C. heterostrophus and C. carbonum were n = 15 or 16 and n = 13 or 15 depending on the strains, respectively. In C. sativus, n = 15 was estimated. Morphological information of chromosomes including chromosome size and a threadlike-specific structure representing the nucleolar organizing region was also obtained. Our results for some standard strains are in agreement with previous estimates by pulsed field gel electrophoresis (PFGE) or PFGE coupled with restriction fragment length polymorphism genetic linkage analysis, but inconsistent with the previous estimates for other strains by conventional light microscopic cytology. Additionally, PFGE analysis of C. heterostrophus strains indicated that chromosome number was not determinable solely by PFGE, which is hampered by comigration and clumping of DNA bands.

Original languageEnglish
Pages (from-to)354-360
Number of pages7
JournalPhytopathology
Volume91
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Genome
  • Northern corn leaf blight
  • Southern corn leaf blight

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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