Abstract
Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.
| Original language | English |
|---|---|
| Pages (from-to) | 254-261 |
| Number of pages | 8 |
| Journal | Journal of General Plant Pathology |
| Volume | 84 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jul 1 2018 |
Keywords
- Chromosome
- Cytology
- Fusarium oxysporum
- Genome
- Karyotype
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science
Fingerprint Dive into the research topics of 'Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)'. Together they form a unique fingerprint.
Cite this
Ayukawa, Y., Komatsu, K., Taga, M., & Arie, T. (2018). Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM). Journal of General Plant Pathology, 84(4), 254-261. https://doi.org/10.1007/s10327-018-0784-5