Abstract
Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.
| Original language | English |
|---|---|
| Pages (from-to) | 254-261 |
| Number of pages | 8 |
| Journal | Journal of General Plant Pathology |
| Volume | 84 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jul 1 2018 |
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Keywords
- Chromosome
- Cytology
- Fusarium oxysporum
- Genome
- Karyotype
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science
Cite this
Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM). / Ayukawa, Yu; Komatsu, Ken; Taga, Masatoki; Arie, Tsutomu.
In: Journal of General Plant Pathology, Vol. 84, No. 4, 01.07.2018, p. 254-261.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)
AU - Ayukawa, Yu
AU - Komatsu, Ken
AU - Taga, Masatoki
AU - Arie, Tsutomu
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.
AB - Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.
KW - Chromosome
KW - Cytology
KW - Fusarium oxysporum
KW - Genome
KW - Karyotype
UR - http://www.scopus.com/inward/record.url?scp=85046892066&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85046892066&partnerID=8YFLogxK
U2 - 10.1007/s10327-018-0784-5
DO - 10.1007/s10327-018-0784-5
M3 - Article
AN - SCOPUS:85046892066
VL - 84
SP - 254
EP - 261
JO - Journal of General Plant Pathology
JF - Journal of General Plant Pathology
SN - 1345-2630
IS - 4
ER -