Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)

Yu Ayukawa, Ken Komatsu, Masatoki Taga, Tsutomu Arie

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.

Original languageEnglish
Pages (from-to)254-261
Number of pages8
JournalJournal of General Plant Pathology
Volume84
Issue number4
DOIs
Publication statusPublished - Jul 1 2018

Fingerprint

germ tube
Fusarium oxysporum
karyotyping
avirulent strains
chromosomes
methodology
DNA probes
fluorescence in situ hybridization
Ascomycota
cell biology
ribosomal DNA
gels
fungi
genome

Keywords

  • Chromosome
  • Cytology
  • Fusarium oxysporum
  • Genome
  • Karyotype

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM). / Ayukawa, Yu; Komatsu, Ken; Taga, Masatoki; Arie, Tsutomu.

In: Journal of General Plant Pathology, Vol. 84, No. 4, 01.07.2018, p. 254-261.

Research output: Contribution to journalArticle

Ayukawa, Yu ; Komatsu, Ken ; Taga, Masatoki ; Arie, Tsutomu. / Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM). In: Journal of General Plant Pathology. 2018 ; Vol. 84, No. 4. pp. 254-261.
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