@article{fb5d770b143345b988dffee0d5910fe1,
title = "Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)",
abstract = "Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.",
keywords = "Chromosome, Cytology, Fusarium oxysporum, Genome, Karyotype",
author = "Yu Ayukawa and Ken Komatsu and Masatoki Taga and Tsutomu Arie",
note = "Funding Information: Acknowledgements This study was partly supported by Grants-in-Aid from The Japan Society for the Promotion of Sciences (JSPS) to TA (16H02536 and 18380030) and Building of Consortia for the Development of Human Resources in Science and Technology (Innovation Advancement Organization, TUAT) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank ASKA Pharmaceutical (Tokyo, Japan) for providing Driselase. We are grateful to Dr. Yoshimiki Amemiya (Chiba University, Matsudo, Japan) for providing a fungal isolate and Dr. Takashi Tsuge (Nagoya University, Nagoya, Japan) for providing plasmid DNA. Publisher Copyright: {\textcopyright} 2018, The Phytopathological Society of Japan and Springer Japan KK, part of Springer Nature.",
year = "2018",
month = jul,
day = "1",
doi = "10.1007/s10327-018-0784-5",
language = "English",
volume = "84",
pages = "254--261",
journal = "Journal of General Plant Pathology",
issn = "1345-2630",
publisher = "Springer Japan",
number = "4",
}