Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)

Yu Ayukawa; Ken Komatsu; Masatoki Taga; Tsutomu Arie

doi:10.1007/s10327-018-0784-5

Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)

Yu Ayukawa, Ken Komatsu, Masatoki Taga, Tsutomu Arie

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.

Original language	English
Pages (from-to)	254-261
Number of pages	8
Journal	Journal of General Plant Pathology
Volume	84
Issue number	4
DOIs	https://doi.org/10.1007/s10327-018-0784-5
Publication status	Published - Jul 1 2018

Keywords

Chromosome
Cytology
Fusarium oxysporum
Genome
Karyotype

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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title = "Cytological karyotyping of Fusarium oxysporum by the germ tube burst method (GTBM)",

abstract = "Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.",

keywords = "Chromosome, Cytology, Fusarium oxysporum, Genome, Karyotype",

author = "Yu Ayukawa and Ken Komatsu and Masatoki Taga and Tsutomu Arie",

note = "Funding Information: Acknowledgements This study was partly supported by Grants-in-Aid from The Japan Society for the Promotion of Sciences (JSPS) to TA (16H02536 and 18380030) and Building of Consortia for the Development of Human Resources in Science and Technology (Innovation Advancement Organization, TUAT) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank ASKA Pharmaceutical (Tokyo, Japan) for providing Driselase. We are grateful to Dr. Yoshimiki Amemiya (Chiba University, Matsudo, Japan) for providing a fungal isolate and Dr. Takashi Tsuge (Nagoya University, Nagoya, Japan) for providing plasmid DNA.",

year = "2018",

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doi = "10.1007/s10327-018-0784-5",

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AU - Ayukawa, Yu

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AU - Taga, Masatoki

AU - Arie, Tsutomu

N1 - Funding Information: Acknowledgements This study was partly supported by Grants-in-Aid from The Japan Society for the Promotion of Sciences (JSPS) to TA (16H02536 and 18380030) and Building of Consortia for the Development of Human Resources in Science and Technology (Innovation Advancement Organization, TUAT) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank ASKA Pharmaceutical (Tokyo, Japan) for providing Driselase. We are grateful to Dr. Yoshimiki Amemiya (Chiba University, Matsudo, Japan) for providing a fungal isolate and Dr. Takashi Tsuge (Nagoya University, Nagoya, Japan) for providing plasmid DNA.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.

AB - Fusarium oxysporum is an ascomycete fungus including plant pathogenic and nonpathogenic strains. Genome analyses have indicated that the karyotype of F. oxysporum is diverse among isolates. Here we used the germ tube burst method (GTBM), a more reliable method than conventional cytology or pulsed field gel electrophoretis, to karyotype isolates of F. oxysporum ff. spp. lycopersici and conglutinans and nonpathogenic F. oxysporum. In this first application of GTBM for F. oxysporum, pathogenic isolates were found to have more chromosomes than in nonpathogenic isolates. We also used a ribosomal DNA probe and fluorescence in situ hybridization (FISH) to analyze chromosome structure.

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