Abstract
Karyotypes of the cucurbit pathogen Nectria haematococca MPI (anamorph Fusarium solani f. sp. cucurbitae race 1) was studied using the two standard strains ATCC18098 and ATCC18099. Complete separation of all chromosomes was difficult with pulsed field gel electrophoresis due to both the large size and co-migration of chromosomes. In contrast, cytological karyotyping was done successfully with fluorescence microscopy combined with the germ tube burst method for sample preparation to visualize mitotic metaphase chromosomes. For each strain the basic chromosome number (CN) was nine, which revises previous chromosome estimates of n = 4. Chromosomes were morphologically characterized by their sizes, intensely fluorescing segments, and protrusion of rDNA. In addition to the basic chromosome complement, ATCC18098 had a mini-chromosome of ~410 kb present as a single copy in somatic nuclei. Chromosome fluorescence in situ hybridization indicated that this mini-chromosome is not a derivative from the other chromosomes in the genome. In addition, crossing experiments suggested that it was transmitted in a Mendelian manner to the ascospore progeny.
| Original language | English |
|---|---|
| Pages (from-to) | 845-856 |
| Number of pages | 12 |
| Journal | Mycologia |
| Volume | 104 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jul 2012 |
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Keywords
- Fluorescence in situ hybridization
- Fusarium solani
- Germ tube burst method
- Minichromosome
- rDNA
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Plant Science
- Cell Biology
- Genetics
- Molecular Biology
- Physiology
Cite this
Cytological karyotyping and characterization of a 410 kb mini-chromosome in nectria haematococca MPI. / Mahmoud, Ahmed M.; Taga, Masatoki.
In: Mycologia, Vol. 104, No. 4, 07.2012, p. 845-856.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytological karyotyping and characterization of a 410 kb mini-chromosome in nectria haematococca MPI
AU - Mahmoud, Ahmed M.
AU - Taga, Masatoki
PY - 2012/7
Y1 - 2012/7
N2 - Karyotypes of the cucurbit pathogen Nectria haematococca MPI (anamorph Fusarium solani f. sp. cucurbitae race 1) was studied using the two standard strains ATCC18098 and ATCC18099. Complete separation of all chromosomes was difficult with pulsed field gel electrophoresis due to both the large size and co-migration of chromosomes. In contrast, cytological karyotyping was done successfully with fluorescence microscopy combined with the germ tube burst method for sample preparation to visualize mitotic metaphase chromosomes. For each strain the basic chromosome number (CN) was nine, which revises previous chromosome estimates of n = 4. Chromosomes were morphologically characterized by their sizes, intensely fluorescing segments, and protrusion of rDNA. In addition to the basic chromosome complement, ATCC18098 had a mini-chromosome of ~410 kb present as a single copy in somatic nuclei. Chromosome fluorescence in situ hybridization indicated that this mini-chromosome is not a derivative from the other chromosomes in the genome. In addition, crossing experiments suggested that it was transmitted in a Mendelian manner to the ascospore progeny.
AB - Karyotypes of the cucurbit pathogen Nectria haematococca MPI (anamorph Fusarium solani f. sp. cucurbitae race 1) was studied using the two standard strains ATCC18098 and ATCC18099. Complete separation of all chromosomes was difficult with pulsed field gel electrophoresis due to both the large size and co-migration of chromosomes. In contrast, cytological karyotyping was done successfully with fluorescence microscopy combined with the germ tube burst method for sample preparation to visualize mitotic metaphase chromosomes. For each strain the basic chromosome number (CN) was nine, which revises previous chromosome estimates of n = 4. Chromosomes were morphologically characterized by their sizes, intensely fluorescing segments, and protrusion of rDNA. In addition to the basic chromosome complement, ATCC18098 had a mini-chromosome of ~410 kb present as a single copy in somatic nuclei. Chromosome fluorescence in situ hybridization indicated that this mini-chromosome is not a derivative from the other chromosomes in the genome. In addition, crossing experiments suggested that it was transmitted in a Mendelian manner to the ascospore progeny.
KW - Fluorescence in situ hybridization
KW - Fusarium solani
KW - Germ tube burst method
KW - Minichromosome
KW - rDNA
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U2 - 10.3852/11-306
DO - 10.3852/11-306
M3 - Article
C2 - 22453120
AN - SCOPUS:84866248562
VL - 104
SP - 845
EP - 856
JO - Mycologia
JF - Mycologia
SN - 0027-5514
IS - 4
ER -