Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica

Ana Eusebio-Cope; Nobuhiro Suzuki; Hamid Sadeghi-Garmaroodi; Masatoki Taga

doi:10.1016/j.fgb.2009.01.005

Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica

Ana Eusebio-Cope, Nobuhiro Suzuki, Hamid Sadeghi-Garmaroodi, Masatoki Taga

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C. parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales.

Original language	English
Pages (from-to)	342-351
Number of pages	10
Journal	Fungal Genetics and Biology
Volume	46
Issue number	4
DOIs	https://doi.org/10.1016/j.fgb.2009.01.005
Publication status	Published - Apr 2009

Keywords

Chromosome
Cryphonectria parasitica
Fluorescent microscopy
Heterochromatin
Hypovirulence
Karyotype
Mitosis
Mycovirus
Plant pathogen

ASJC Scopus subject areas

Microbiology
Genetics

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title = "Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica",

abstract = "The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C. parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales.",

keywords = "Chromosome, Cryphonectria parasitica, Fluorescent microscopy, Heterochromatin, Hypovirulence, Karyotype, Mitosis, Mycovirus, Plant pathogen",

author = "Ana Eusebio-Cope and Nobuhiro Suzuki and Hamid Sadeghi-Garmaroodi and Masatoki Taga",

note = "Funding Information: We thank Donald L. Nuss and Bradley I. Hillman for the generous gift of fungal strains and their helpful discussion. This work was supported in part by the Okayama University COE program {\textquoteleft}Establishment of Plant Health Science{\textquoteright} to NS and MT and by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-Oriented Industries to NS. ",

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AU - Sadeghi-Garmaroodi, Hamid

AU - Taga, Masatoki

N1 - Funding Information: We thank Donald L. Nuss and Bradley I. Hillman for the generous gift of fungal strains and their helpful discussion. This work was supported in part by the Okayama University COE program ‘Establishment of Plant Health Science’ to NS and MT and by the Program for Promotion of Basic and Applied Researches for Innovations in Bio-Oriented Industries to NS.

PY - 2009/4

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N2 - The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C. parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales.

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KW - Mycovirus

KW - Plant pathogen

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Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica

Abstract

Keywords

ASJC Scopus subject areas

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