Abstract
The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C. parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales.
| Original language | English |
|---|---|
| Pages (from-to) | 342-351 |
| Number of pages | 10 |
| Journal | Fungal Genetics and Biology |
| Volume | 46 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 2009 |
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Keywords
- Chromosome
- Cryphonectria parasitica
- Fluorescent microscopy
- Heterochromatin
- Hypovirulence
- Karyotype
- Mitosis
- Mycovirus
- Plant pathogen
ASJC Scopus subject areas
- Genetics
- Microbiology
Cite this
Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica. / Eusebio-Cope, Ana; Suzuki, Nobuhiro; Sadeghi-Garmaroodi, Hamid; Taga, Masatoki.
In: Fungal Genetics and Biology, Vol. 46, No. 4, 04.2009, p. 342-351.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica
AU - Eusebio-Cope, Ana
AU - Suzuki, Nobuhiro
AU - Sadeghi-Garmaroodi, Hamid
AU - Taga, Masatoki
PY - 2009/4
Y1 - 2009/4
N2 - The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C. parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales.
AB - The karyotypes of nine strains including three transformants of the chestnut blight fungus Cryphonectria parasitica were analyzed by pulsed-field gel electrophoresis (PFGE) and cytology using a fluorescence microscope. Cytology of the mitotic metaphase showed n = 9 for both standard strain EP155 and field strain GH2 infected by Cryphonectria hypovirus 3. Chromosomes were morphologically characterized by size, heterochromatic segment, and constriction. PFGE resolved 5 or 6 chromosomal DNA bands ranging from 3.3 Mbp to 9.7 Mbp, but accurate determination of the chromosome number was hampered by clumping of some bands. Banding profiles in PFGE were similar among the strains except for GH2, in which a chromosome translocation was detected by Southern blot analysis. By integrating the data from cytology and PFGE, the genome size of C. parasitica was estimated to be ca. 50 Mbp. This is the first report of a cytological karyotype in the order Diaporthales.
KW - Chromosome
KW - Cryphonectria parasitica
KW - Fluorescent microscopy
KW - Heterochromatin
KW - Hypovirulence
KW - Karyotype
KW - Mitosis
KW - Mycovirus
KW - Plant pathogen
UR - http://www.scopus.com/inward/record.url?scp=61449206996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=61449206996&partnerID=8YFLogxK
U2 - 10.1016/j.fgb.2009.01.005
DO - 10.1016/j.fgb.2009.01.005
M3 - Article
C2 - 19570503
AN - SCOPUS:61449206996
VL - 46
SP - 342
EP - 351
JO - Fungal Genetics and Biology
JF - Fungal Genetics and Biology
SN - 1087-1845
IS - 4
ER -