Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene

Hiroki Ukitsu, Takashi Kuromori, Kiminori Toyooka, Yumi Goto, Ken Matsuoka, Eiji Sakuradani, Sakayu Shimizu, Asako Kamiya, Yuko Imura, Masahiro Yuguchi, Takuji Wada, Takashi Hirayama, Kazuo Shinozaki

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves and infertility. Toluidine blue testing and scanning electron microscopy observation revealed that these mutants had cuticular defects in the stems and adult leaves, but not in cotyledones. Transmission electron microscopy observation revealed thinner cuticle layers in the mutants, and cuticular materials interspersed between the two fused epidermal layers were observed in the mutant rosette leaves. These two mutants had a transposon insertion in the coding regions of WBC11, which was classified as a member of ABC transporter genes in Arabidopsis. WBC11 showed high sequence similarity to CER5 (also called WBC12), which was involved in cuticular lipid export. Gas chromatographic analysis revealed that C29 alkane extracted from the stem surface of cof1 mutants was reduced whereas C29 ketone was accumulated, which was different from the case of cer5 mutants. While cer5 mutants had fairly normal morphology, cof1 mutants had pleiotropic phenotypes so that COF1/WBC11 could have important roles different from those of CER5/WBC12. We also found that C29 alkane was accumulated in the intracellular extract of cof1 mutants, suggesting a function for WBC11 in the direct transport of lipid molecules. Pollen observation showed that mutant pollen grains were irregularly shaped. The function of COF1/WBC11 in lipid transport for the construction of cuticle layers and pollen coats for normal organ formation is discussed.

Original languageEnglish
Pages (from-to)1524-1533
Number of pages10
JournalPlant and Cell Physiology
Volume48
Issue number11
DOIs
Publication statusPublished - Nov 2007
Externally publishedYes

Fingerprint

ABC transporters
ATP-Binding Cassette Transporters
Pollen
Arabidopsis
Alkanes
Observation
Lipids
mutants
Genes
Phenotype
Tolonium Chloride
genes
Ketones
Transmission Electron Microscopy
Gas Chromatography
Electron Scanning Microscopy
Infertility
Soil
pollen
transposons

Keywords

  • ABC transporter
  • Arabidopsis thaliana
  • Cuticle
  • Organ fusion
  • Transposon-tagged line

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Ukitsu, H., Kuromori, T., Toyooka, K., Goto, Y., Matsuoka, K., Sakuradani, E., ... Shinozaki, K. (2007). Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene. Plant and Cell Physiology, 48(11), 1524-1533. https://doi.org/10.1093/pcp/pcm139

Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene. / Ukitsu, Hiroki; Kuromori, Takashi; Toyooka, Kiminori; Goto, Yumi; Matsuoka, Ken; Sakuradani, Eiji; Shimizu, Sakayu; Kamiya, Asako; Imura, Yuko; Yuguchi, Masahiro; Wada, Takuji; Hirayama, Takashi; Shinozaki, Kazuo.

In: Plant and Cell Physiology, Vol. 48, No. 11, 11.2007, p. 1524-1533.

Research output: Contribution to journalArticle

Ukitsu, H, Kuromori, T, Toyooka, K, Goto, Y, Matsuoka, K, Sakuradani, E, Shimizu, S, Kamiya, A, Imura, Y, Yuguchi, M, Wada, T, Hirayama, T & Shinozaki, K 2007, 'Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene', Plant and Cell Physiology, vol. 48, no. 11, pp. 1524-1533. https://doi.org/10.1093/pcp/pcm139
Ukitsu H, Kuromori T, Toyooka K, Goto Y, Matsuoka K, Sakuradani E et al. Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene. Plant and Cell Physiology. 2007 Nov;48(11):1524-1533. https://doi.org/10.1093/pcp/pcm139
Ukitsu, Hiroki ; Kuromori, Takashi ; Toyooka, Kiminori ; Goto, Yumi ; Matsuoka, Ken ; Sakuradani, Eiji ; Shimizu, Sakayu ; Kamiya, Asako ; Imura, Yuko ; Yuguchi, Masahiro ; Wada, Takuji ; Hirayama, Takashi ; Shinozaki, Kazuo. / Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene. In: Plant and Cell Physiology. 2007 ; Vol. 48, No. 11. pp. 1524-1533.
@article{806e0daaa1064562910bd82a3410707b,
title = "Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene",
abstract = "In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves and infertility. Toluidine blue testing and scanning electron microscopy observation revealed that these mutants had cuticular defects in the stems and adult leaves, but not in cotyledones. Transmission electron microscopy observation revealed thinner cuticle layers in the mutants, and cuticular materials interspersed between the two fused epidermal layers were observed in the mutant rosette leaves. These two mutants had a transposon insertion in the coding regions of WBC11, which was classified as a member of ABC transporter genes in Arabidopsis. WBC11 showed high sequence similarity to CER5 (also called WBC12), which was involved in cuticular lipid export. Gas chromatographic analysis revealed that C29 alkane extracted from the stem surface of cof1 mutants was reduced whereas C29 ketone was accumulated, which was different from the case of cer5 mutants. While cer5 mutants had fairly normal morphology, cof1 mutants had pleiotropic phenotypes so that COF1/WBC11 could have important roles different from those of CER5/WBC12. We also found that C29 alkane was accumulated in the intracellular extract of cof1 mutants, suggesting a function for WBC11 in the direct transport of lipid molecules. Pollen observation showed that mutant pollen grains were irregularly shaped. The function of COF1/WBC11 in lipid transport for the construction of cuticle layers and pollen coats for normal organ formation is discussed.",
keywords = "ABC transporter, Arabidopsis thaliana, Cuticle, Organ fusion, Transposon-tagged line",
author = "Hiroki Ukitsu and Takashi Kuromori and Kiminori Toyooka and Yumi Goto and Ken Matsuoka and Eiji Sakuradani and Sakayu Shimizu and Asako Kamiya and Yuko Imura and Masahiro Yuguchi and Takuji Wada and Takashi Hirayama and Kazuo Shinozaki",
year = "2007",
month = "11",
doi = "10.1093/pcp/pcm139",
language = "English",
volume = "48",
pages = "1524--1533",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "11",

}

TY - JOUR

T1 - Cytological and biochemical analysis of COF1, an Arabidopsis mutant of an ABC transporter gene

AU - Ukitsu, Hiroki

AU - Kuromori, Takashi

AU - Toyooka, Kiminori

AU - Goto, Yumi

AU - Matsuoka, Ken

AU - Sakuradani, Eiji

AU - Shimizu, Sakayu

AU - Kamiya, Asako

AU - Imura, Yuko

AU - Yuguchi, Masahiro

AU - Wada, Takuji

AU - Hirayama, Takashi

AU - Shinozaki, Kazuo

PY - 2007/11

Y1 - 2007/11

N2 - In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves and infertility. Toluidine blue testing and scanning electron microscopy observation revealed that these mutants had cuticular defects in the stems and adult leaves, but not in cotyledones. Transmission electron microscopy observation revealed thinner cuticle layers in the mutants, and cuticular materials interspersed between the two fused epidermal layers were observed in the mutant rosette leaves. These two mutants had a transposon insertion in the coding regions of WBC11, which was classified as a member of ABC transporter genes in Arabidopsis. WBC11 showed high sequence similarity to CER5 (also called WBC12), which was involved in cuticular lipid export. Gas chromatographic analysis revealed that C29 alkane extracted from the stem surface of cof1 mutants was reduced whereas C29 ketone was accumulated, which was different from the case of cer5 mutants. While cer5 mutants had fairly normal morphology, cof1 mutants had pleiotropic phenotypes so that COF1/WBC11 could have important roles different from those of CER5/WBC12. We also found that C29 alkane was accumulated in the intracellular extract of cof1 mutants, suggesting a function for WBC11 in the direct transport of lipid molecules. Pollen observation showed that mutant pollen grains were irregularly shaped. The function of COF1/WBC11 in lipid transport for the construction of cuticle layers and pollen coats for normal organ formation is discussed.

AB - In transposon-tagged lines of Arabidopsis we found two new mutants, cof1-1 and cof1-2 (cuticular defect and organ fusion), that show the phenotype of wilting when grown in soil, organ fusion of rosette leaves and infertility. Toluidine blue testing and scanning electron microscopy observation revealed that these mutants had cuticular defects in the stems and adult leaves, but not in cotyledones. Transmission electron microscopy observation revealed thinner cuticle layers in the mutants, and cuticular materials interspersed between the two fused epidermal layers were observed in the mutant rosette leaves. These two mutants had a transposon insertion in the coding regions of WBC11, which was classified as a member of ABC transporter genes in Arabidopsis. WBC11 showed high sequence similarity to CER5 (also called WBC12), which was involved in cuticular lipid export. Gas chromatographic analysis revealed that C29 alkane extracted from the stem surface of cof1 mutants was reduced whereas C29 ketone was accumulated, which was different from the case of cer5 mutants. While cer5 mutants had fairly normal morphology, cof1 mutants had pleiotropic phenotypes so that COF1/WBC11 could have important roles different from those of CER5/WBC12. We also found that C29 alkane was accumulated in the intracellular extract of cof1 mutants, suggesting a function for WBC11 in the direct transport of lipid molecules. Pollen observation showed that mutant pollen grains were irregularly shaped. The function of COF1/WBC11 in lipid transport for the construction of cuticle layers and pollen coats for normal organ formation is discussed.

KW - ABC transporter

KW - Arabidopsis thaliana

KW - Cuticle

KW - Organ fusion

KW - Transposon-tagged line

UR - http://www.scopus.com/inward/record.url?scp=36249001591&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=36249001591&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcm139

DO - 10.1093/pcp/pcm139

M3 - Article

C2 - 17971336

AN - SCOPUS:36249001591

VL - 48

SP - 1524

EP - 1533

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 11

ER -