Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung

Fumiharu Yanagi, Hidenori Terasaki, Akihiro Matsukawa, Susumu Ohkawara, Tohru Morioka, Masaru Yoshinaga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

To examine host responses to extracorporeal lung assist (ECLA) in small animals, we developed a mini hollow fiber lung of nonmicroporous polyolefin and an extracorporeal bypass circuit with a priming volume of 25 ml. This circuit allowed ECLA of up to 72 h without blood transfusion in 20 rabbits. The ECLA procedure induced the appearance of tumor necrosis factor-a (TNF-α) and interleukin-1 (IL-1) receptor antagonist (IL-1Ra) in plasma, but not IL-1β. However, these changes were observed only at the initial stage of ECLA, and the levels returned to pre-ECLA levels within 24 h. Although leukocytes adhering to the hollow fibers were immunohistochemically positive for IL-1β and IL-1Ra, the plasma levels of these cytokines in response to ECLA were not different from those observed in rabbits given anesthesia and subjected to minor surgery but without ECLA. Thus, ECLA itself is a minor factor in the production of these cytokines.

Original languageEnglish
Pages (from-to)209-217
Number of pages9
JournalArtificial Organs
Volume20
Issue number3
Publication statusPublished - 1996
Externally publishedYes

Fingerprint

Interleukin-1 Receptors
Interleukin-1
Cytokines
Rabbits
Plasmas
Lung
Networks (circuits)
Fibers
Polyolefins
Surgery
Animals
Blood
Tumor Necrosis Factor-alpha
Minor Surgical Procedures
Blood Transfusion
Leukocytes
Anesthesia
PL 732

Keywords

  • Cardiopulmonary bypass
  • Cytokines
  • Extracorporeal lung assist
  • Extracorporeal membrane oxygenation
  • Interleukin-1 receptor antagonist
  • Interleukin-1β
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Biophysics

Cite this

Yanagi, F., Terasaki, H., Matsukawa, A., Ohkawara, S., Morioka, T., & Yoshinaga, M. (1996). Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung. Artificial Organs, 20(3), 209-217.

Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung. / Yanagi, Fumiharu; Terasaki, Hidenori; Matsukawa, Akihiro; Ohkawara, Susumu; Morioka, Tohru; Yoshinaga, Masaru.

In: Artificial Organs, Vol. 20, No. 3, 1996, p. 209-217.

Research output: Contribution to journalArticle

Yanagi, F, Terasaki, H, Matsukawa, A, Ohkawara, S, Morioka, T & Yoshinaga, M 1996, 'Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung', Artificial Organs, vol. 20, no. 3, pp. 209-217.
Yanagi, Fumiharu ; Terasaki, Hidenori ; Matsukawa, Akihiro ; Ohkawara, Susumu ; Morioka, Tohru ; Yoshinaga, Masaru. / Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung. In: Artificial Organs. 1996 ; Vol. 20, No. 3. pp. 209-217.
@article{a92d5c6f85704350978cf986fe8f2649,
title = "Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung",
abstract = "To examine host responses to extracorporeal lung assist (ECLA) in small animals, we developed a mini hollow fiber lung of nonmicroporous polyolefin and an extracorporeal bypass circuit with a priming volume of 25 ml. This circuit allowed ECLA of up to 72 h without blood transfusion in 20 rabbits. The ECLA procedure induced the appearance of tumor necrosis factor-a (TNF-α) and interleukin-1 (IL-1) receptor antagonist (IL-1Ra) in plasma, but not IL-1β. However, these changes were observed only at the initial stage of ECLA, and the levels returned to pre-ECLA levels within 24 h. Although leukocytes adhering to the hollow fibers were immunohistochemically positive for IL-1β and IL-1Ra, the plasma levels of these cytokines in response to ECLA were not different from those observed in rabbits given anesthesia and subjected to minor surgery but without ECLA. Thus, ECLA itself is a minor factor in the production of these cytokines.",
keywords = "Cardiopulmonary bypass, Cytokines, Extracorporeal lung assist, Extracorporeal membrane oxygenation, Interleukin-1 receptor antagonist, Interleukin-1β, Tumor necrosis factor-α",
author = "Fumiharu Yanagi and Hidenori Terasaki and Akihiro Matsukawa and Susumu Ohkawara and Tohru Morioka and Masaru Yoshinaga",
year = "1996",
language = "English",
volume = "20",
pages = "209--217",
journal = "Artificial Organs",
issn = "0160-564X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Cytokine generation in rabbits during extracorporeal lung assist with a mini hollow fiber lung

AU - Yanagi, Fumiharu

AU - Terasaki, Hidenori

AU - Matsukawa, Akihiro

AU - Ohkawara, Susumu

AU - Morioka, Tohru

AU - Yoshinaga, Masaru

PY - 1996

Y1 - 1996

N2 - To examine host responses to extracorporeal lung assist (ECLA) in small animals, we developed a mini hollow fiber lung of nonmicroporous polyolefin and an extracorporeal bypass circuit with a priming volume of 25 ml. This circuit allowed ECLA of up to 72 h without blood transfusion in 20 rabbits. The ECLA procedure induced the appearance of tumor necrosis factor-a (TNF-α) and interleukin-1 (IL-1) receptor antagonist (IL-1Ra) in plasma, but not IL-1β. However, these changes were observed only at the initial stage of ECLA, and the levels returned to pre-ECLA levels within 24 h. Although leukocytes adhering to the hollow fibers were immunohistochemically positive for IL-1β and IL-1Ra, the plasma levels of these cytokines in response to ECLA were not different from those observed in rabbits given anesthesia and subjected to minor surgery but without ECLA. Thus, ECLA itself is a minor factor in the production of these cytokines.

AB - To examine host responses to extracorporeal lung assist (ECLA) in small animals, we developed a mini hollow fiber lung of nonmicroporous polyolefin and an extracorporeal bypass circuit with a priming volume of 25 ml. This circuit allowed ECLA of up to 72 h without blood transfusion in 20 rabbits. The ECLA procedure induced the appearance of tumor necrosis factor-a (TNF-α) and interleukin-1 (IL-1) receptor antagonist (IL-1Ra) in plasma, but not IL-1β. However, these changes were observed only at the initial stage of ECLA, and the levels returned to pre-ECLA levels within 24 h. Although leukocytes adhering to the hollow fibers were immunohistochemically positive for IL-1β and IL-1Ra, the plasma levels of these cytokines in response to ECLA were not different from those observed in rabbits given anesthesia and subjected to minor surgery but without ECLA. Thus, ECLA itself is a minor factor in the production of these cytokines.

KW - Cardiopulmonary bypass

KW - Cytokines

KW - Extracorporeal lung assist

KW - Extracorporeal membrane oxygenation

KW - Interleukin-1 receptor antagonist

KW - Interleukin-1β

KW - Tumor necrosis factor-α

UR - http://www.scopus.com/inward/record.url?scp=0029927946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029927946&partnerID=8YFLogxK

M3 - Article

C2 - 8694691

AN - SCOPUS:0029927946

VL - 20

SP - 209

EP - 217

JO - Artificial Organs

JF - Artificial Organs

SN - 0160-564X

IS - 3

ER -