Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array

Takamaro Sato, Yoshihiko Soga, Tomoko Yamaguchi, Michio Meguro, Hiroshi Maeda, Joji Tada, Takayuki Otani, Masaharu Seno, Shogo Takashiba

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown. Objective: The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array. Methods: The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array. Results: ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/ Scheffe's test, P <0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation. Conclusion: ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation.

Original languageEnglish
Pages (from-to)271-276
Number of pages6
JournalAsian Pacific Journal of Allergy and Immunology
Volume31
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Eosinophil Cationic Protein
Protein Array Analysis
Fibroblasts
Cytokines
Skin
Growth
Ciliary Neurotrophic Factor
Neurotrophin 3
Inflammation
Stress Fibers
Peptides
Cardiac Myocytes
Analysis of Variance
Cell Death
Cell Count

Keywords

  • Cytokine
  • Eosinophil cationic protein
  • Fibroblast
  • Growth

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array. / Sato, Takamaro; Soga, Yoshihiko; Yamaguchi, Tomoko; Meguro, Michio; Maeda, Hiroshi; Tada, Joji; Otani, Takayuki; Seno, Masaharu; Takashiba, Shogo.

In: Asian Pacific Journal of Allergy and Immunology, Vol. 31, No. 4, 2013, p. 271-276.

Research output: Contribution to journalArticle

Sato, Takamaro ; Soga, Yoshihiko ; Yamaguchi, Tomoko ; Meguro, Michio ; Maeda, Hiroshi ; Tada, Joji ; Otani, Takayuki ; Seno, Masaharu ; Takashiba, Shogo. / Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array. In: Asian Pacific Journal of Allergy and Immunology. 2013 ; Vol. 31, No. 4. pp. 271-276.
@article{d345f489106d4505909fcab2463e1dc3,
title = "Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array",
abstract = "Background: Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown. Objective: The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array. Methods: The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array. Results: ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/ Scheffe's test, P <0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation. Conclusion: ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation.",
keywords = "Cytokine, Eosinophil cationic protein, Fibroblast, Growth",
author = "Takamaro Sato and Yoshihiko Soga and Tomoko Yamaguchi and Michio Meguro and Hiroshi Maeda and Joji Tada and Takayuki Otani and Masaharu Seno and Shogo Takashiba",
year = "2013",
doi = "10.12932/AP0287.31.4.2013",
language = "English",
volume = "31",
pages = "271--276",
journal = "Asian Pacific Journal of Allergy and Immunology",
issn = "0125-877X",
publisher = "The Allergy and Immunology Society of Thailand",
number = "4",

}

TY - JOUR

T1 - Cytokine expression in human dermal fibroblasts stimulated with eosinophil cationic protein measured by protein array

AU - Sato, Takamaro

AU - Soga, Yoshihiko

AU - Yamaguchi, Tomoko

AU - Meguro, Michio

AU - Maeda, Hiroshi

AU - Tada, Joji

AU - Otani, Takayuki

AU - Seno, Masaharu

AU - Takashiba, Shogo

PY - 2013

Y1 - 2013

N2 - Background: Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown. Objective: The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array. Methods: The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array. Results: ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/ Scheffe's test, P <0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation. Conclusion: ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation.

AB - Background: Eosinophil cationic protein (ECP) was reported previously to be involved in allergic inflammation with cytotoxic activity. On the other hand, recent studies showed that ECP did not induce cell death but inhibited the growth of cancer-derived cells. Our previous study indicated that human ECP enhanced differentiation of rat neonatal cardiomyocytes and stress fiber formation in Balb/c 3T3 mouse fibroblasts, while the effects of human ECP on human fibroblasts are unknown. Objective: The present study was performed to determine the effects of human ECP on cytokine expression in human fibroblasts by protein array. Methods: The effects of recombinant human ECP (rhECP) on normal human dermal fibroblasts (NHDF) were examined by assaying cell growth. Furthermore, cytokine expression of NHDF stimulated by ECP, which could influence cell growth, was evaluated by protein array. Results: ECP was not cytotoxic but enhanced the growth of NHDF. The peak rhECP concentration that enhanced the cell counts by 1.56-fold was 100 ng/mL, which was significantly different from cultures without ECP stimulation (ANOVA/ Scheffe's test, P <0.05). Array analyses indicated that ciliary neurotrophic factor (CNTF), neutrophilactivating peptide (NAP)-2, and neurotrophin (NT)-3 were significantly upregulated in NHDF stimulated with 100 ng/mL ECP compared to those without stimulation. Conclusion: ECP is not cytotoxic but enhances the growth of NHDF. CNTF, NAP-2, and NT-3 were suggested to be involved in enhancing the growth of NHDF. These findings will contribute to determination of the role of ECP in allergic inflammation.

KW - Cytokine

KW - Eosinophil cationic protein

KW - Fibroblast

KW - Growth

UR - http://www.scopus.com/inward/record.url?scp=84897019107&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897019107&partnerID=8YFLogxK

U2 - 10.12932/AP0287.31.4.2013

DO - 10.12932/AP0287.31.4.2013

M3 - Article

VL - 31

SP - 271

EP - 276

JO - Asian Pacific Journal of Allergy and Immunology

JF - Asian Pacific Journal of Allergy and Immunology

SN - 0125-877X

IS - 4

ER -