Cytokine dialysis: An ex vivo study

Shigehiko Uchino, Rinaldo Bellomo, Hiroshi Morimatsu, Donna Goldsmith, Piers Davenport, Louise Cole, Ian Baldwin, Sianna Panagiotopoulos, Peter Tipping, Stanislao Morgera, Hans Hellmut Neumayer, Hermann Goehl

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

To test the hypothesis that dialysis using a new large pore membrane would achieve effective cytokine removal, blood from six volunteers was incubated with endotoxin (1 mg) and then circulated through a closed circuit with a polyamide membrane (nominal cut-off: 100 kDa). Hemodialysis was conducted at 1 or 9 L/hr of dialysate flow at the start of circulation and after 2 and 4 hours. The peak dialysate/plasma concentration ratios were 0.92 for interleukin (IL)-1β, 0.67 for IL-6, 0.94 for IL-8, 0.33 for tumor necrosis factor (TNF)-α, and 0.11 for albumin. The dialysate/plasma ratios for all cytokines and albumin were decreased with increased dialysate flow from 1 to 9 L/hr (p <0.05). Clearances for IL-1β, IL-6, and IL-8, however, were significantly improved with increased dialysate flow (p <0.01). There was no increase in TNF-α clearance (not significant) and a decrease in albumin clearance (p <0.01). The peak clearance at 9 L/hr was 33 ml/min for IL-1β, 19 for IL-6, 51 for IL-8, 11 for TNF-α, and 1.2 for albumin. No adsorption of cytokines was observed. We conclude that cytokine dialysis is achievable through a membrane with a high cut-off point with negligible albumin loss. These findings support the technical feasibility of this new approach to blood purification in sepsis.

Original languageEnglish
Pages (from-to)650-653
Number of pages4
JournalASAIO Journal
Volume48
Issue number6
Publication statusPublished - Nov 2002
Externally publishedYes

Fingerprint

Dialysis
Dialysis Solutions
Albumins
Cytokines
Interleukin-8
Interleukin-1
Interleukin-6
Membranes
Tumor Necrosis Factor-alpha
Blood
Plasmas
Interleukin-11
Polyamides
Purification
Nylons
Endotoxins
Adsorption
Renal Dialysis
Volunteers
Sepsis

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering

Cite this

Uchino, S., Bellomo, R., Morimatsu, H., Goldsmith, D., Davenport, P., Cole, L., ... Goehl, H. (2002). Cytokine dialysis: An ex vivo study. ASAIO Journal, 48(6), 650-653.

Cytokine dialysis : An ex vivo study. / Uchino, Shigehiko; Bellomo, Rinaldo; Morimatsu, Hiroshi; Goldsmith, Donna; Davenport, Piers; Cole, Louise; Baldwin, Ian; Panagiotopoulos, Sianna; Tipping, Peter; Morgera, Stanislao; Neumayer, Hans Hellmut; Goehl, Hermann.

In: ASAIO Journal, Vol. 48, No. 6, 11.2002, p. 650-653.

Research output: Contribution to journalArticle

Uchino, S, Bellomo, R, Morimatsu, H, Goldsmith, D, Davenport, P, Cole, L, Baldwin, I, Panagiotopoulos, S, Tipping, P, Morgera, S, Neumayer, HH & Goehl, H 2002, 'Cytokine dialysis: An ex vivo study', ASAIO Journal, vol. 48, no. 6, pp. 650-653.
Uchino S, Bellomo R, Morimatsu H, Goldsmith D, Davenport P, Cole L et al. Cytokine dialysis: An ex vivo study. ASAIO Journal. 2002 Nov;48(6):650-653.
Uchino, Shigehiko ; Bellomo, Rinaldo ; Morimatsu, Hiroshi ; Goldsmith, Donna ; Davenport, Piers ; Cole, Louise ; Baldwin, Ian ; Panagiotopoulos, Sianna ; Tipping, Peter ; Morgera, Stanislao ; Neumayer, Hans Hellmut ; Goehl, Hermann. / Cytokine dialysis : An ex vivo study. In: ASAIO Journal. 2002 ; Vol. 48, No. 6. pp. 650-653.
@article{7fc6aebef6e04adfa7bb9c58e6162b34,
title = "Cytokine dialysis: An ex vivo study",
abstract = "To test the hypothesis that dialysis using a new large pore membrane would achieve effective cytokine removal, blood from six volunteers was incubated with endotoxin (1 mg) and then circulated through a closed circuit with a polyamide membrane (nominal cut-off: 100 kDa). Hemodialysis was conducted at 1 or 9 L/hr of dialysate flow at the start of circulation and after 2 and 4 hours. The peak dialysate/plasma concentration ratios were 0.92 for interleukin (IL)-1β, 0.67 for IL-6, 0.94 for IL-8, 0.33 for tumor necrosis factor (TNF)-α, and 0.11 for albumin. The dialysate/plasma ratios for all cytokines and albumin were decreased with increased dialysate flow from 1 to 9 L/hr (p <0.05). Clearances for IL-1β, IL-6, and IL-8, however, were significantly improved with increased dialysate flow (p <0.01). There was no increase in TNF-α clearance (not significant) and a decrease in albumin clearance (p <0.01). The peak clearance at 9 L/hr was 33 ml/min for IL-1β, 19 for IL-6, 51 for IL-8, 11 for TNF-α, and 1.2 for albumin. No adsorption of cytokines was observed. We conclude that cytokine dialysis is achievable through a membrane with a high cut-off point with negligible albumin loss. These findings support the technical feasibility of this new approach to blood purification in sepsis.",
author = "Shigehiko Uchino and Rinaldo Bellomo and Hiroshi Morimatsu and Donna Goldsmith and Piers Davenport and Louise Cole and Ian Baldwin and Sianna Panagiotopoulos and Peter Tipping and Stanislao Morgera and Neumayer, {Hans Hellmut} and Hermann Goehl",
year = "2002",
month = "11",
language = "English",
volume = "48",
pages = "650--653",
journal = "ASAIO Journal",
issn = "1058-2916",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Cytokine dialysis

T2 - An ex vivo study

AU - Uchino, Shigehiko

AU - Bellomo, Rinaldo

AU - Morimatsu, Hiroshi

AU - Goldsmith, Donna

AU - Davenport, Piers

AU - Cole, Louise

AU - Baldwin, Ian

AU - Panagiotopoulos, Sianna

AU - Tipping, Peter

AU - Morgera, Stanislao

AU - Neumayer, Hans Hellmut

AU - Goehl, Hermann

PY - 2002/11

Y1 - 2002/11

N2 - To test the hypothesis that dialysis using a new large pore membrane would achieve effective cytokine removal, blood from six volunteers was incubated with endotoxin (1 mg) and then circulated through a closed circuit with a polyamide membrane (nominal cut-off: 100 kDa). Hemodialysis was conducted at 1 or 9 L/hr of dialysate flow at the start of circulation and after 2 and 4 hours. The peak dialysate/plasma concentration ratios were 0.92 for interleukin (IL)-1β, 0.67 for IL-6, 0.94 for IL-8, 0.33 for tumor necrosis factor (TNF)-α, and 0.11 for albumin. The dialysate/plasma ratios for all cytokines and albumin were decreased with increased dialysate flow from 1 to 9 L/hr (p <0.05). Clearances for IL-1β, IL-6, and IL-8, however, were significantly improved with increased dialysate flow (p <0.01). There was no increase in TNF-α clearance (not significant) and a decrease in albumin clearance (p <0.01). The peak clearance at 9 L/hr was 33 ml/min for IL-1β, 19 for IL-6, 51 for IL-8, 11 for TNF-α, and 1.2 for albumin. No adsorption of cytokines was observed. We conclude that cytokine dialysis is achievable through a membrane with a high cut-off point with negligible albumin loss. These findings support the technical feasibility of this new approach to blood purification in sepsis.

AB - To test the hypothesis that dialysis using a new large pore membrane would achieve effective cytokine removal, blood from six volunteers was incubated with endotoxin (1 mg) and then circulated through a closed circuit with a polyamide membrane (nominal cut-off: 100 kDa). Hemodialysis was conducted at 1 or 9 L/hr of dialysate flow at the start of circulation and after 2 and 4 hours. The peak dialysate/plasma concentration ratios were 0.92 for interleukin (IL)-1β, 0.67 for IL-6, 0.94 for IL-8, 0.33 for tumor necrosis factor (TNF)-α, and 0.11 for albumin. The dialysate/plasma ratios for all cytokines and albumin were decreased with increased dialysate flow from 1 to 9 L/hr (p <0.05). Clearances for IL-1β, IL-6, and IL-8, however, were significantly improved with increased dialysate flow (p <0.01). There was no increase in TNF-α clearance (not significant) and a decrease in albumin clearance (p <0.01). The peak clearance at 9 L/hr was 33 ml/min for IL-1β, 19 for IL-6, 51 for IL-8, 11 for TNF-α, and 1.2 for albumin. No adsorption of cytokines was observed. We conclude that cytokine dialysis is achievable through a membrane with a high cut-off point with negligible albumin loss. These findings support the technical feasibility of this new approach to blood purification in sepsis.

UR - http://www.scopus.com/inward/record.url?scp=0036839901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036839901&partnerID=8YFLogxK

M3 - Article

C2 - 12455777

AN - SCOPUS:0036839901

VL - 48

SP - 650

EP - 653

JO - ASAIO Journal

JF - ASAIO Journal

SN - 1058-2916

IS - 6

ER -