Cytokine-dependent synergistic regulation of interleukin-8 production from human gingival fibroblasts

M. Takigawa, Shogo Takashiba, F. Myokai, K. Takahashi, H. Arai, H. Kurihara, Y. Murayama

Research output: Contribution to journalArticle

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Abstract

Human gingival fibroblasts (HGF) may have an important role in the orchestration of immuno-participant cells infiltrating the gingiva in response to continuously recurring bacterial infection. To examine the cytokine network regulating HGF-derived interleukin (IL)-8, a potent neutrophil chemotactic cytokine, we analyzed the effects of inflammatory cytokines alone and in combination on IL-8 production by HGF. IL-1β, tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-6, and IL-8 were used as stimulants. HGF secreted IL-8 in a dose-dependent manner after stimulation with either IL-1β or TNF-α, but not with IFN-γ or IL-6. Furthermore, IL-8 itself did not affect IL-8 mRNA accumulation in HGF in an autocrine manner. The combination of IL-1β and TNF-α synergistically enhanced the secretion of IL-8, whereas IFN-γ suppressed IL-8 secretion by IL-1β- or TNF-α-stimulated HGF. These effects were also observed at each level of IL-8 mRNA expression in HGF. IL-8 secretion by cytokine-stimulated HGF was not influenced by the inhibition of PGE2 synthesis with indomethacin, indicating that endogenous PGE2 was not involved in IL-8 production by HGF. These results indicate that IL-8 production by HGF is synergistically stimulated by specific cytokines, IL-1β and TNF-α, and suggest that these stimulatory effects are down-regulated by IFN-γ at the transcriptional level through PGE2-independent pathways. Thus, neutrophil-mediated processes in periodontal disease may be regulated in part by HGF in the cytokine network of immuno-participant cells.

Original languageEnglish
Pages (from-to)1002-1007
Number of pages6
JournalJournal of Periodontology
Volume65
Issue number11
Publication statusPublished - 1994

Fingerprint

Interleukin-8
Fibroblasts
Cytokines
Interleukin-1
Interferons
Tumor Necrosis Factor-alpha
Dinoprostone
Interleukin-6
Neutrophils
Messenger RNA
Gingiva
Periodontal Diseases
Chemokines
Bacterial Infections
Indomethacin

Keywords

  • bacterial infections
  • cytokines
  • fibroblasts
  • interleukin-8
  • neutrophils
  • synergism

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Takigawa, M., Takashiba, S., Myokai, F., Takahashi, K., Arai, H., Kurihara, H., & Murayama, Y. (1994). Cytokine-dependent synergistic regulation of interleukin-8 production from human gingival fibroblasts. Journal of Periodontology, 65(11), 1002-1007.

Cytokine-dependent synergistic regulation of interleukin-8 production from human gingival fibroblasts. / Takigawa, M.; Takashiba, Shogo; Myokai, F.; Takahashi, K.; Arai, H.; Kurihara, H.; Murayama, Y.

In: Journal of Periodontology, Vol. 65, No. 11, 1994, p. 1002-1007.

Research output: Contribution to journalArticle

Takigawa, M, Takashiba, S, Myokai, F, Takahashi, K, Arai, H, Kurihara, H & Murayama, Y 1994, 'Cytokine-dependent synergistic regulation of interleukin-8 production from human gingival fibroblasts', Journal of Periodontology, vol. 65, no. 11, pp. 1002-1007.
Takigawa, M. ; Takashiba, Shogo ; Myokai, F. ; Takahashi, K. ; Arai, H. ; Kurihara, H. ; Murayama, Y. / Cytokine-dependent synergistic regulation of interleukin-8 production from human gingival fibroblasts. In: Journal of Periodontology. 1994 ; Vol. 65, No. 11. pp. 1002-1007.
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