Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres

Yuki Shirosaki, Manato Nakatsukasa, Saki Yasutomi, Susana Cruz-Neves, Satoshi Hayakawa, Akiyoshi Osaka, Toshinari Maeda, Toshiki Miyazaki

Research output: Contribution to journalArticle

Abstract

Microporous spheres in a hybrid system consisting of chitosan and γ-glycidoxypropyltrimethoxysilane (GPTMS) have advantages in a range of applications, e.g., as vehicles for cell transplantation and soft tissue defect filling materials, because of their excellent cytocompatibility with various cells. In this study, microporous chitosan-GPTMS spheres were prepared by dropping chitosan-GPTMS precursor sols,with orwithout a ceriumchloride, into liquid nitrogen using a syringe pump. The droplets were then freeze dried to give the pores of size 10 to 50 μm. The cell culture tests showed that L929 fibroblast-like cells migrated into the micropores larger than 50 μm in diameter, whereasMG63 osteoblast-like cells proliferated well and covered the granule surfaces. The spheres with cerium chloride showed antibacterial properties against both gram-negative and gram-positive bacteria.

Original languageEnglish
Article number1676
JournalPolymers
Volume11
Issue number10
DOIs
Publication statusPublished - Oct 1 2019

Fingerprint

Siloxanes
Chitosan
Cerium
Syringes
Osteoblasts
Polymethyl Methacrylate
Liquid nitrogen
Fibroblasts
Sols
Hybrid systems
Cell culture
Chlorides
Bacteria
Pumps
Tissue
Defects

Keywords

  • Antibacterial property
  • Cell behavior
  • Cerium ion
  • Chitosan-siloxane hybrid
  • Microporous spheres

ASJC Scopus subject areas

  • Chemistry(all)
  • Polymers and Plastics

Cite this

Shirosaki, Y., Nakatsukasa, M., Yasutomi, S., Cruz-Neves, S., Hayakawa, S., Osaka, A., ... Miyazaki, T. (2019). Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres. Polymers, 11(10), [1676]. https://doi.org/10.3390/polym11101676

Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres. / Shirosaki, Yuki; Nakatsukasa, Manato; Yasutomi, Saki; Cruz-Neves, Susana; Hayakawa, Satoshi; Osaka, Akiyoshi; Maeda, Toshinari; Miyazaki, Toshiki.

In: Polymers, Vol. 11, No. 10, 1676, 01.10.2019.

Research output: Contribution to journalArticle

Shirosaki, Y, Nakatsukasa, M, Yasutomi, S, Cruz-Neves, S, Hayakawa, S, Osaka, A, Maeda, T & Miyazaki, T 2019, 'Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres', Polymers, vol. 11, no. 10, 1676. https://doi.org/10.3390/polym11101676
Shirosaki Y, Nakatsukasa M, Yasutomi S, Cruz-Neves S, Hayakawa S, Osaka A et al. Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres. Polymers. 2019 Oct 1;11(10). 1676. https://doi.org/10.3390/polym11101676
Shirosaki, Yuki ; Nakatsukasa, Manato ; Yasutomi, Saki ; Cruz-Neves, Susana ; Hayakawa, Satoshi ; Osaka, Akiyoshi ; Maeda, Toshinari ; Miyazaki, Toshiki. / Cytocompatible and antibacterial properties of chitosan-siloxane hybrid spheres. In: Polymers. 2019 ; Vol. 11, No. 10.
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