Cysteine dioxygenase and γ-glutamylcysteine synthetase activities in primary cultured hepatocytes respond to sulfur amino acid supplementation in a reciprocal manner

Jun Ohta, Y. H. Kwon, M. H. Stipanuk

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hepatocytes were cultured for 3 days as spheroids (aggregates) or as monolayers in basal medium and in sulfur amino acid-supplemented media. Cultured hepatocytes had low levels of cysteine dioxygenase (CDO) activity and normal levels of γ-glutamylcysteine synthetase (GCS) and cysteine-sulfinate decarboxylase (CSDC) activities compared to freshly isolated cells. CDO activity increased and GCS activity decreased in a dose-response manner in cells cultured in either methionine- or cysteine-supplemented media. CSDC activity was not significantly affected by methionine supplementation. Changes in CDO and GCS were associated with changes in cysteine catabolism to taurine plus sulfate and in synthesis of glutathione, respectively. These responses are similar to those observed in liver of intact rats fed diets supplemented with sulfur amino acids. A near-maximal response of CDO or GCS activity was observed when the medium contained 1.0mmol/L of methionine plus cyst(e)ine. Changes in CDO and GCS activities did not appear to be mediated by changes in the intracellular glutathione concentration. Cultured hepatocytes offer a useful model for further studies of cysteine metabolism and its regulation in response to sulfur amino acid availability.

Original languageEnglish
Pages (from-to)705-728
Number of pages24
JournalAmino Acids
Volume19
Issue number3-4
Publication statusPublished - 2000

Fingerprint

Cysteine Dioxygenase
Glutamate-Cysteine Ligase
Sulfur Amino Acids
Hepatocytes
sulfoalanine decarboxylase
Methionine
Cysteine
Glutathione
Taurine
Nutrition
Metabolism
Liver
Sulfates
Cysts
Rats
Cultured Cells
Monolayers
Availability
Diet

Keywords

  • γ-Glutamylcysteine synthetase
  • Amino acids
  • Cysteine
  • Cysteine dioxygenase
  • Glutathione
  • Hepatocytes
  • Taurine

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry
  • Endocrinology

Cite this

Cysteine dioxygenase and γ-glutamylcysteine synthetase activities in primary cultured hepatocytes respond to sulfur amino acid supplementation in a reciprocal manner. / Ohta, Jun; Kwon, Y. H.; Stipanuk, M. H.

In: Amino Acids, Vol. 19, No. 3-4, 2000, p. 705-728.

Research output: Contribution to journalArticle

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