Cysteine biosynthesis in Saccharomyces cerevisiae: A new outlook on pathway and regulation

Bun Ichiro Ono, Toshiya Hazu, Sayaka Yoshida, Takahiro Kawato, Sumio Shinoda, Jerzy Brzvwczy, Andrzej Paszewski

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Using a Saccharomyces cerevisiae strain having the activities of serine O-acetyl-transferase (SATase), O-acetylserine/O-acetylhomoserine sulphydrylase (OAS/OAH SHLase), cystathionine β-synthase (β-CTSase) and cystathionine γ-lyase (γ-CTLase), we individually disrupted CYS3 (coding for γ-CTLase) and CYS4 (coding for β-CTSase). The obtained gene disruptants were cysteine-dependent and incorporated the radioactivity of 35S-sulphate into homocysteine but not into cysteine or glutathione. We concluded, therefore, that SATase and OAS/OAH SHLase do not constitute a cysteine biosynthetic pathway and that cysteine is synthesized exclusively through the pathway constituted with β-CTSase and γ-CTLase; note that OAS/OAH SHLase supplies homocysteine to this pathway by acting as OAH SHLase. From further investigation upon the cys3-disruptant, we obtained results consistent with our earlier suggestion that cysteine and OAS play central roles in the regulation of sulphate assimilation. In addition, we found that sulphate transport activity was not induced at all in the cys4-disruptant, suggesting that CYS4 plays a role in the regulation of sulphate assimilation.

Original languageEnglish
Pages (from-to)1365-1375
Number of pages11
JournalYeast
Volume15
Issue number13
DOIs
Publication statusPublished - Sep 30 1999

Fingerprint

Biosynthesis
Yeast
Cysteine
cysteine
Saccharomyces cerevisiae
Sulfates
biosynthesis
sulfates
serine O-acetyltransferase
Cystathionine
cystathionine
homocysteine
Homocysteine
Transferases
Serine
assimilation (physiology)
Radioactivity
Lyases
lyases
Biosynthetic Pathways

Keywords

  • Cysteine biosynthesis
  • Gene disruption
  • Saccharomyces cerevisiae
  • Sulphate assimilation

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering
  • Microbiology

Cite this

Ono, B. I., Hazu, T., Yoshida, S., Kawato, T., Shinoda, S., Brzvwczy, J., & Paszewski, A. (1999). Cysteine biosynthesis in Saccharomyces cerevisiae: A new outlook on pathway and regulation. Yeast, 15(13), 1365-1375. https://doi.org/10.1002/(SICI)1097-0061(19990930)15:13<1365::AID-YEA468>3.0.CO;2-U

Cysteine biosynthesis in Saccharomyces cerevisiae : A new outlook on pathway and regulation. / Ono, Bun Ichiro; Hazu, Toshiya; Yoshida, Sayaka; Kawato, Takahiro; Shinoda, Sumio; Brzvwczy, Jerzy; Paszewski, Andrzej.

In: Yeast, Vol. 15, No. 13, 30.09.1999, p. 1365-1375.

Research output: Contribution to journalArticle

Ono, BI, Hazu, T, Yoshida, S, Kawato, T, Shinoda, S, Brzvwczy, J & Paszewski, A 1999, 'Cysteine biosynthesis in Saccharomyces cerevisiae: A new outlook on pathway and regulation', Yeast, vol. 15, no. 13, pp. 1365-1375. https://doi.org/10.1002/(SICI)1097-0061(19990930)15:13<1365::AID-YEA468>3.0.CO;2-U
Ono, Bun Ichiro ; Hazu, Toshiya ; Yoshida, Sayaka ; Kawato, Takahiro ; Shinoda, Sumio ; Brzvwczy, Jerzy ; Paszewski, Andrzej. / Cysteine biosynthesis in Saccharomyces cerevisiae : A new outlook on pathway and regulation. In: Yeast. 1999 ; Vol. 15, No. 13. pp. 1365-1375.
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