Cystathionine γ-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine γ-lyase in filamentous bacteria and its purification and characterization

T. Nagasawa, Hiroshi Kanzaki, H. Yamada

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Abstract

Cystathionine γ-lyase (EC 4.4.1.1) is widely distributed in actinomycetes, e.g. genera Streptomyces, Micromonospora, Micropolyspora, Mycobacterium, Nocardia, Streptosporangium, and Streptoverticillium. The enzyme was purified from Streptomyces phaeochromogenes (IFO 3105) in nine steps. After the last steps, the enzyme appeared to be homogenous by the criteria of polyacrylamide gel electrophoresis, analytical centrifugation, and double diffusion in agarose. The enzyme crystallized in the apo form with the addition of ammonium sulfate. The enzyme has a molecular weight of about 166,000 and consists of four subunits identical in molecular weight. The enzyme exhibits absorption maxima at 278 and 421 nm and contains 4 mol of pyridoxal 5'-phosphate/mol of enzyme. L-Cystathionine, L-homoserine, DL-lanthionine, L-djenkolic acid, and L-cysteine are cleaved as preferred substrates by the Streptomyces enzyme. The α,β-elimination reaction of L-cystathionine is also catalyzed by the enzyme at a ratio of about one-seventh of the α,γ-elimination reaction. Cystathionine β-synthase (EC 4.2.1.22) and cystathionine γ-synthase (EC 4.2.99.9) activities were also detected in crude extracts of S. phaeochromogenes, but cystathionine β-lyase (EC 4.4.1.8) was not. Consequently, the reverse transsulfuration pathway in actinomycetes may be similar to that in yeast and molds.

Original languageEnglish
Pages (from-to)10393-10403
Number of pages11
JournalJournal of Biological Chemistry
Volume259
Issue number16
Publication statusPublished - 1984
Externally publishedYes

Fingerprint

Cystathionine
Lyases
Streptomyces
Purification
Bacteria
Enzymes
Nocardia
Actinobacteria
cystathionine beta-lyase
Molecular Weight
Micromonospora
Molecular weight
Homoserine
Pyridoxal Phosphate
Centrifugation
Ammonium Sulfate
Molds
Mycobacterium
Electrophoresis
Complex Mixtures

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cystathionine γ-lyase of Streptomyces phaeochromogenes. The occurrence of cystathionine γ-lyase in filamentous bacteria and its purification and characterization. / Nagasawa, T.; Kanzaki, Hiroshi; Yamada, H.

In: Journal of Biological Chemistry, Vol. 259, No. 16, 1984, p. 10393-10403.

Research output: Contribution to journalArticle

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