Cyclooxygenase 2 expression in otitis media with effusion

Masashi Kakiuchi, Hidetsugu Tsujigiwa, Yorihisa Orita, Hitoshi Nagatsuka, Junko Yoshinobu, Shin Kariya, Shin Ichi Haginomori, Saeko Orita, Kunihiro Fukushima, Mitsuhiro Okano, Noriyuki Nagai, Kazunori Nishizaki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Objectives: To study whether cyclooxygenase 2 (COX-2) plays a role in the development of otitis media with effusion (OME). Study design/methods: An experimental model of endotoxin-induced OME was used in healthy BALB/c mice. Solutions of Salmonella typhimurium endotoxin were injected into the middle ears of the mice, and COX-2 expression in the middle ears was studied using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Results: Analysis by RT-PCR showed that COX-2 expression in the middle ear increased in a dose-dependent fashion after injection of endotoxin. ISH demonstrated that COX-2 had distinct expression patterns in the epithelium of middle ears with endotoxin-induced OME. Conclusions: COX-2 expression, however, was not detected in normal middle ears. COX-2 expression may be one factor contributing to the development of OME.

Original languageEnglish
Pages (from-to)81-85
Number of pages5
JournalAmerican Journal of Otolaryngology - Head and Neck Medicine and Surgery
Volume27
Issue number2
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Otitis Media with Effusion
Middle Ear
Cyclooxygenase 2
Endotoxins
Reverse Transcription
In Situ Hybridization
Polymerase Chain Reaction
Salmonella typhimurium
Theoretical Models
Epithelium
Injections

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Cyclooxygenase 2 expression in otitis media with effusion. / Kakiuchi, Masashi; Tsujigiwa, Hidetsugu; Orita, Yorihisa; Nagatsuka, Hitoshi; Yoshinobu, Junko; Kariya, Shin; Haginomori, Shin Ichi; Orita, Saeko; Fukushima, Kunihiro; Okano, Mitsuhiro; Nagai, Noriyuki; Nishizaki, Kazunori.

In: American Journal of Otolaryngology - Head and Neck Medicine and Surgery, Vol. 27, No. 2, 03.2006, p. 81-85.

Research output: Contribution to journalArticle

Kakiuchi, M, Tsujigiwa, H, Orita, Y, Nagatsuka, H, Yoshinobu, J, Kariya, S, Haginomori, SI, Orita, S, Fukushima, K, Okano, M, Nagai, N & Nishizaki, K 2006, 'Cyclooxygenase 2 expression in otitis media with effusion', American Journal of Otolaryngology - Head and Neck Medicine and Surgery, vol. 27, no. 2, pp. 81-85. https://doi.org/10.1016/j.amjoto.2005.07.009
Kakiuchi, Masashi ; Tsujigiwa, Hidetsugu ; Orita, Yorihisa ; Nagatsuka, Hitoshi ; Yoshinobu, Junko ; Kariya, Shin ; Haginomori, Shin Ichi ; Orita, Saeko ; Fukushima, Kunihiro ; Okano, Mitsuhiro ; Nagai, Noriyuki ; Nishizaki, Kazunori. / Cyclooxygenase 2 expression in otitis media with effusion. In: American Journal of Otolaryngology - Head and Neck Medicine and Surgery. 2006 ; Vol. 27, No. 2. pp. 81-85.
@article{5f11cc916d5240389bfb9872e00c40d9,
title = "Cyclooxygenase 2 expression in otitis media with effusion",
abstract = "Objectives: To study whether cyclooxygenase 2 (COX-2) plays a role in the development of otitis media with effusion (OME). Study design/methods: An experimental model of endotoxin-induced OME was used in healthy BALB/c mice. Solutions of Salmonella typhimurium endotoxin were injected into the middle ears of the mice, and COX-2 expression in the middle ears was studied using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Results: Analysis by RT-PCR showed that COX-2 expression in the middle ear increased in a dose-dependent fashion after injection of endotoxin. ISH demonstrated that COX-2 had distinct expression patterns in the epithelium of middle ears with endotoxin-induced OME. Conclusions: COX-2 expression, however, was not detected in normal middle ears. COX-2 expression may be one factor contributing to the development of OME.",
author = "Masashi Kakiuchi and Hidetsugu Tsujigiwa and Yorihisa Orita and Hitoshi Nagatsuka and Junko Yoshinobu and Shin Kariya and Haginomori, {Shin Ichi} and Saeko Orita and Kunihiro Fukushima and Mitsuhiro Okano and Noriyuki Nagai and Kazunori Nishizaki",
year = "2006",
month = "3",
doi = "10.1016/j.amjoto.2005.07.009",
language = "English",
volume = "27",
pages = "81--85",
journal = "American Journal of Otolaryngology - Head and Neck Medicine and Surgery",
issn = "0196-0709",
publisher = "W.B. Saunders Ltd",
number = "2",

}

TY - JOUR

T1 - Cyclooxygenase 2 expression in otitis media with effusion

AU - Kakiuchi, Masashi

AU - Tsujigiwa, Hidetsugu

AU - Orita, Yorihisa

AU - Nagatsuka, Hitoshi

AU - Yoshinobu, Junko

AU - Kariya, Shin

AU - Haginomori, Shin Ichi

AU - Orita, Saeko

AU - Fukushima, Kunihiro

AU - Okano, Mitsuhiro

AU - Nagai, Noriyuki

AU - Nishizaki, Kazunori

PY - 2006/3

Y1 - 2006/3

N2 - Objectives: To study whether cyclooxygenase 2 (COX-2) plays a role in the development of otitis media with effusion (OME). Study design/methods: An experimental model of endotoxin-induced OME was used in healthy BALB/c mice. Solutions of Salmonella typhimurium endotoxin were injected into the middle ears of the mice, and COX-2 expression in the middle ears was studied using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Results: Analysis by RT-PCR showed that COX-2 expression in the middle ear increased in a dose-dependent fashion after injection of endotoxin. ISH demonstrated that COX-2 had distinct expression patterns in the epithelium of middle ears with endotoxin-induced OME. Conclusions: COX-2 expression, however, was not detected in normal middle ears. COX-2 expression may be one factor contributing to the development of OME.

AB - Objectives: To study whether cyclooxygenase 2 (COX-2) plays a role in the development of otitis media with effusion (OME). Study design/methods: An experimental model of endotoxin-induced OME was used in healthy BALB/c mice. Solutions of Salmonella typhimurium endotoxin were injected into the middle ears of the mice, and COX-2 expression in the middle ears was studied using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization (ISH). Results: Analysis by RT-PCR showed that COX-2 expression in the middle ear increased in a dose-dependent fashion after injection of endotoxin. ISH demonstrated that COX-2 had distinct expression patterns in the epithelium of middle ears with endotoxin-induced OME. Conclusions: COX-2 expression, however, was not detected in normal middle ears. COX-2 expression may be one factor contributing to the development of OME.

UR - http://www.scopus.com/inward/record.url?scp=33144460235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33144460235&partnerID=8YFLogxK

U2 - 10.1016/j.amjoto.2005.07.009

DO - 10.1016/j.amjoto.2005.07.009

M3 - Article

C2 - 16500468

AN - SCOPUS:33144460235

VL - 27

SP - 81

EP - 85

JO - American Journal of Otolaryngology - Head and Neck Medicine and Surgery

JF - American Journal of Otolaryngology - Head and Neck Medicine and Surgery

SN - 0196-0709

IS - 2

ER -