Abstract
Methyl jasmonate (MeJA) signalling shares several signal components with abscisic acid (ABA) signalling in guard cells. Cyclic adenosine 5'-diphosphoribose (cADPR) and cyclic guanosine 3',5'-monophosphate (cGMP) are second messengers in ABA-induced stomatal closure. In order to clarify involvement of cADPR and cGMP in MeJA-induced stomatal closure in Arabidopsis thaliana (Col-0), we investigated effects of an inhibitor of cADPR synthesis, nicotinamide (NA), and an inhibitor of cGMP synthesis, LY83583 (LY, 6-anilino-5,8-quinolinedione), on MeJA-induced stomatal closure. Treatment with NA and LY inhibited MeJA-induced stomatal closure. NA inhibited MeJA-induced reactive oxygen species (ROS) accumulation and nitric oxide (NO) production in guard cells. NA and LY suppressed transient elevations elicited by MeJA in cytosolic free Ca2+ concentration ([Ca2+]cyt) in guard cells. These results suggest that cADPR and cGMP positively function in [Ca2+]cyt elevation in MeJA-induced stomatal closure, are signalling components shared with ABA-induced stomatal closure in Arabidopsis, and that cADPR is required for MeJA-induced ROS accumulation and NO production in Arabidopsis guard cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1140-1144 |
| Number of pages | 5 |
| Journal | Plant Biology |
| Volume | 16 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 1 2014 |
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Keywords
- Abscisic acid
- Arabidopsis thaliana
- Cyclic adenosine 5'-diphosphoribose
- Cyclic guanosine 3',5'-monophosphate
- Methyl jasmonate
ASJC Scopus subject areas
- Plant Science
- Ecology, Evolution, Behavior and Systematics
- Medicine(all)
Cite this
Cyclic adenosine 5'-diphosphoribose (cADPR) cyclic guanosine 3',5'-monophosphate positively function in Ca2+ elevation in methyl jasmonate-induced stomatal closure, cADPR is required for methyl jasmonate-induced ROS accumulation NO production in guard cells. / Hossain, M. A.; Ye, W.; Munemasa, Shintaro; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.
In: Plant Biology, Vol. 16, No. 6, 01.11.2014, p. 1140-1144.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Cyclic adenosine 5'-diphosphoribose (cADPR) cyclic guanosine 3',5'-monophosphate positively function in Ca2+ elevation in methyl jasmonate-induced stomatal closure, cADPR is required for methyl jasmonate-induced ROS accumulation NO production in guard cells
AU - Hossain, M. A.
AU - Ye, W.
AU - Munemasa, Shintaro
AU - Nakamura, Yoshimasa
AU - Mori, Izumi
AU - Murata, Yoshiyuki
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Methyl jasmonate (MeJA) signalling shares several signal components with abscisic acid (ABA) signalling in guard cells. Cyclic adenosine 5'-diphosphoribose (cADPR) and cyclic guanosine 3',5'-monophosphate (cGMP) are second messengers in ABA-induced stomatal closure. In order to clarify involvement of cADPR and cGMP in MeJA-induced stomatal closure in Arabidopsis thaliana (Col-0), we investigated effects of an inhibitor of cADPR synthesis, nicotinamide (NA), and an inhibitor of cGMP synthesis, LY83583 (LY, 6-anilino-5,8-quinolinedione), on MeJA-induced stomatal closure. Treatment with NA and LY inhibited MeJA-induced stomatal closure. NA inhibited MeJA-induced reactive oxygen species (ROS) accumulation and nitric oxide (NO) production in guard cells. NA and LY suppressed transient elevations elicited by MeJA in cytosolic free Ca2+ concentration ([Ca2+]cyt) in guard cells. These results suggest that cADPR and cGMP positively function in [Ca2+]cyt elevation in MeJA-induced stomatal closure, are signalling components shared with ABA-induced stomatal closure in Arabidopsis, and that cADPR is required for MeJA-induced ROS accumulation and NO production in Arabidopsis guard cells.
AB - Methyl jasmonate (MeJA) signalling shares several signal components with abscisic acid (ABA) signalling in guard cells. Cyclic adenosine 5'-diphosphoribose (cADPR) and cyclic guanosine 3',5'-monophosphate (cGMP) are second messengers in ABA-induced stomatal closure. In order to clarify involvement of cADPR and cGMP in MeJA-induced stomatal closure in Arabidopsis thaliana (Col-0), we investigated effects of an inhibitor of cADPR synthesis, nicotinamide (NA), and an inhibitor of cGMP synthesis, LY83583 (LY, 6-anilino-5,8-quinolinedione), on MeJA-induced stomatal closure. Treatment with NA and LY inhibited MeJA-induced stomatal closure. NA inhibited MeJA-induced reactive oxygen species (ROS) accumulation and nitric oxide (NO) production in guard cells. NA and LY suppressed transient elevations elicited by MeJA in cytosolic free Ca2+ concentration ([Ca2+]cyt) in guard cells. These results suggest that cADPR and cGMP positively function in [Ca2+]cyt elevation in MeJA-induced stomatal closure, are signalling components shared with ABA-induced stomatal closure in Arabidopsis, and that cADPR is required for MeJA-induced ROS accumulation and NO production in Arabidopsis guard cells.
KW - Abscisic acid
KW - Arabidopsis thaliana
KW - Cyclic adenosine 5'-diphosphoribose
KW - Cyclic guanosine 3',5'-monophosphate
KW - Methyl jasmonate
UR - http://www.scopus.com/inward/record.url?scp=84926122901&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84926122901&partnerID=8YFLogxK
U2 - 10.1111/plb.12175
DO - 10.1111/plb.12175
M3 - Article
C2 - 24802616
AN - SCOPUS:84926122901
VL - 16
SP - 1140
EP - 1144
JO - Acta Botanica Neerlandica
JF - Acta Botanica Neerlandica
SN - 1435-8603
IS - 6
ER -