Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection

Kenzo Fujimoto; Yoshinaga Yoshimura; Makoto Ihara; Kazuhiko Matsuda; Yuko Takeuchi; Takaaki Aoki; Toru Ide

doi:10.1016/j.bmcl.2007.12.002

Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection

Kenzo Fujimoto, Yoshinaga Yoshimura, Makoto Ihara, Kazuhiko Matsuda, Yuko Takeuchi, Takaaki Aoki, Toru Ide

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.

Original language	English
Pages (from-to)	1106-1109
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/j.bmcl.2007.12.002
Publication status	Published - Feb 1 2008
Externally published	Yes

Keywords

Fluorescence
Ion channels
Receptors
Single molecule studies

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/j.bmcl.2007.12.002

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abstract = "We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.",

keywords = "Fluorescence, Ion channels, Receptors, Single molecule studies",

author = "Kenzo Fujimoto and Yoshinaga Yoshimura and Makoto Ihara and Kazuhiko Matsuda and Yuko Takeuchi and Takaaki Aoki and Toru Ide",

note = "Funding Information: The authors thank Dr. Peter Karagiannis for carefully revising the manuscript. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Molecular and System Life Science, and Innovative nanoscience of supermolecular motor proteins working in biomembranes). ",

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T1 - Cy3-3-acylcholine

T2 - A fluorescent analogue of acetylcholine for single molecule detection

AU - Fujimoto, Kenzo

AU - Yoshimura, Yoshinaga

AU - Ihara, Makoto

AU - Matsuda, Kazuhiko

AU - Takeuchi, Yuko

AU - Aoki, Takaaki

AU - Ide, Toru

N1 - Funding Information: The authors thank Dr. Peter Karagiannis for carefully revising the manuscript. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Molecular and System Life Science, and Innovative nanoscience of supermolecular motor proteins working in biomembranes).

PY - 2008/2/1

Y1 - 2008/2/1

N2 - We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.

AB - We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.

KW - Fluorescence

KW - Ion channels

KW - Receptors

KW - Single molecule studies

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ER -

Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection

Abstract

Keywords

ASJC Scopus subject areas

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