Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection

Kenzo Fujimoto; Yoshinaga Yoshimura; Makoto Ihara; Kazuhiko Matsuda; Yuko Takeuchi; Takaaki Aoki; Toru Ide

doi:10.1016/j.bmcl.2007.12.002

Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection

Kenzo Fujimoto, Yoshinaga Yoshimura, Makoto Ihara, Kazuhiko Matsuda, Yuko Takeuchi, Takaaki Aoki, Toru Ide

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.

Original language	English
Pages (from-to)	1106-1109
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	18
Issue number	3
DOIs	https://doi.org/10.1016/j.bmcl.2007.12.002
Publication status	Published - Feb 1 2008
Externally published	Yes

Keywords

Fluorescence
Ion channels
Receptors
Single molecule studies

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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T2 - A fluorescent analogue of acetylcholine for single molecule detection

AU - Fujimoto, Kenzo

AU - Yoshimura, Yoshinaga

AU - Ihara, Makoto

AU - Matsuda, Kazuhiko

AU - Takeuchi, Yuko

AU - Aoki, Takaaki

AU - Ide, Toru

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KW - Ion channels

KW - Receptors

KW - Single molecule studies

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