Cy3-3-acylcholine: A fluorescent analogue of acetylcholine for single molecule detection

Kenzo Fujimoto, Yoshinaga Yoshimura, Makoto Ihara, Kazuhiko Matsuda, Yuko Takeuchi, Takaaki Aoki, Toru Ide

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We synthesized a novel fluorescent analogue of acetylcholine, Cy3-3-acylcholine. The molecular weight of the products agreed with structural predictions. Discrete intensity changes of fluorescent spots due to a single ligand binding/unbinding to nAChR were visualized by TIRF microscopy. The agonist effect of the Cy3-3-acylcholine on nicotinic acetylcholine receptor (nAChR) was confirmed electrophysiologically. This newly synthesized fluorescent analogue will enable us to conduct more elaborate studies on single channel interaction processes between nAChR and ligands.

Original languageEnglish
Pages (from-to)1106-1109
Number of pages4
JournalBioorganic and Medicinal Chemistry Letters
Volume18
Issue number3
DOIs
Publication statusPublished - Feb 1 2008
Externally publishedYes

Keywords

  • Fluorescence
  • Ion channels
  • Receptors
  • Single molecule studies

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

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