CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence

Shunsuke Numata, Makiko Nagata, Han Mao, Kazuhisa Sekimizu, Chikara Kaito

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We previously identified CvfA (SA1129) as a Staphylococcus aureus virulence factor using a silkworm infection model. S. aureus cvfA-deleted mutants exhibit decreased expression of the agr locus encoding a positive regulator of hemolysin genes and decreased hemolysin production. CvfA protein hydrolyzes a 2′,3′-cyclic phosphodiester bond at the RNA 3′ terminus, producing RNA with a 3′-phosphate (3′-phosphorylated RNA, RNAwith a 3′-phosphate). Here, we report that the cvfA-deleted mutant phenotype (decreased agr expression and hemolysin production) was suppressed by disrupting pnpA-encoding polynucleotide phosphorylase (PNPase) with 3′- to 5′-exonuclease activity. The suppression was blocked by introducing a pnpA-encoding PNPase with exonuclease activity but not by a pnpA-encoding mutant PNPase without exonuclease activity. Therefore, loss of PNPase exonuclease activity suppressed the cvfA-deleted mutant phenotype. Purified PNPase efficiently degraded RNA with 2′,3′-cyclic phosphate at the 3′ terminus (2′,3′-cyclic RNA), but it inefficiently degraded 3′-phosphorylated RNA. These findings indicate that 3′- phosphorylated RNA production from 2′,3′-cyclic RNA by CvfA prevents RNA degradation by PNPase and contributes to the expression of agr and hemolysin genes. We speculate that in the cvfA-deleted mutant, 2′,3′-cyclic RNA is not converted to the 3′-phosphorylated form and is efficiently degraded by PNPase, resulting in the loss of RNA essential for expressing agr and hemolysin genes, whereas in the cvfA/pnpA double-disrupted mutant, 2′,3′-cyclic RNA is not degraded by PNPase, leading to hemolysin production. These findings suggest that CvfA and PNPase competitively regulate RNA degradation essential for S. aureus virulence.

Original languageEnglish
Pages (from-to)8420-8431
Number of pages12
JournalJournal of Biological Chemistry
Volume289
Issue number12
DOIs
Publication statusPublished - Mar 21 2014
Externally publishedYes

Fingerprint

Polyribonucleotide Nucleotidyltransferase
Virulence
Staphylococcus aureus
RNA
Hemolysin Proteins
Exonucleases
Proteins
Phosphates
RNA Stability
Genes
Phenotype
Phosphodiesterase I
Bombyx
Degradation
Virulence Factors
Regulator Genes

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence. / Numata, Shunsuke; Nagata, Makiko; Mao, Han; Sekimizu, Kazuhisa; Kaito, Chikara.

In: Journal of Biological Chemistry, Vol. 289, No. 12, 21.03.2014, p. 8420-8431.

Research output: Contribution to journalArticle

Numata, Shunsuke ; Nagata, Makiko ; Mao, Han ; Sekimizu, Kazuhisa ; Kaito, Chikara. / CvfA protein and polynucleotide phosphorylase act in an opposing manner to regulate Staphylococcus aureus virulence. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 12. pp. 8420-8431.
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