Cutting edge: Critical role of IκB kinaseain TLR7/9-induced type I IFN production by conventional dendritic cells

Katsuaki Hoshino, Izumi Sasaki, Takahiro Sugiyama, Takahiro Yano, Chihiro Yamazaki, Teruhito Yasui, Hitoshi Kikutani, Tsuneyasu Kaisho

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A plasmacytoid dendritic cell (DC) can produce large amounts of type I IFNs after sensing nucleic acids through TLR7 and TLR9. IκB kinase α (IKKα) is critically involved in this type I IFN production through its interaction with IFN regulatory factor-7. In response to TLR7/9 signaling, conventional DCs can also produce IFN-β but not IFN-α in a type I IFN-independent manner. In this study, we showed that IKKα was required for production of IFN-β, but not of proinflammatory cytokines, by TLR7/9-stimulated conventional DCs. Importantly, IKKα was dispensable for IFN-β gene upregulation by TLR4 signaling. Biochemical analyses indicated that IKKα exerted its effects through its interaction with IFN regulatory factor-1. Furthermore, IKKα was involved in TLR9-induced type I IFN-independent IFN-β production in vivo. Our results show that IKKα is a uniquemolecule involved in TLR7/9-MyD88-dependent type I IFN production through DC subset-specific mechanisms.

Original languageEnglish
Pages (from-to)3341-3345
Number of pages5
JournalJournal of Immunology
Volume184
Issue number7
DOIs
Publication statusPublished - Apr 1 2010
Externally publishedYes

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Dendritic Cells
Nucleic Acids
Phosphotransferases
Up-Regulation
Cytokines
Genes

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Hoshino, K., Sasaki, I., Sugiyama, T., Yano, T., Yamazaki, C., Yasui, T., ... Kaisho, T. (2010). Cutting edge: Critical role of IκB kinaseain TLR7/9-induced type I IFN production by conventional dendritic cells. Journal of Immunology, 184(7), 3341-3345. https://doi.org/10.4049/jimmunol.0901648

Cutting edge : Critical role of IκB kinaseain TLR7/9-induced type I IFN production by conventional dendritic cells. / Hoshino, Katsuaki; Sasaki, Izumi; Sugiyama, Takahiro; Yano, Takahiro; Yamazaki, Chihiro; Yasui, Teruhito; Kikutani, Hitoshi; Kaisho, Tsuneyasu.

In: Journal of Immunology, Vol. 184, No. 7, 01.04.2010, p. 3341-3345.

Research output: Contribution to journalArticle

Hoshino, K, Sasaki, I, Sugiyama, T, Yano, T, Yamazaki, C, Yasui, T, Kikutani, H & Kaisho, T 2010, 'Cutting edge: Critical role of IκB kinaseain TLR7/9-induced type I IFN production by conventional dendritic cells', Journal of Immunology, vol. 184, no. 7, pp. 3341-3345. https://doi.org/10.4049/jimmunol.0901648
Hoshino, Katsuaki ; Sasaki, Izumi ; Sugiyama, Takahiro ; Yano, Takahiro ; Yamazaki, Chihiro ; Yasui, Teruhito ; Kikutani, Hitoshi ; Kaisho, Tsuneyasu. / Cutting edge : Critical role of IκB kinaseain TLR7/9-induced type I IFN production by conventional dendritic cells. In: Journal of Immunology. 2010 ; Vol. 184, No. 7. pp. 3341-3345.
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