Current recordings of ion channel proteins immobilized on resin beads

Minako Hirano, Yuko Takeuchi, Takaaki Aoki, Toshio Yanagida, Toru Ide

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Current ion channel current measurement techniques are cumbersome, as they require many steps and much time. This is especially true when reconstituting channels into liposomes and incorporating them into lipid bilayers. Here, we report a novel method that measures ion channel current more efficiently than current methods. We applied our method to KcsA and MthK channels by binding them to cobalt affinity gel beads with histidine tags and then forming a lipid bilayer membrane on the bead. This allowed channels to incorporate into the bilayer and channel currents to be measured quickly and easily. The efficiency was such that currents could be recorded with extremely low amounts of protein. In addition, the channel direction could be determined by the histidine tag. This method has the potential to be applied to various channel proteins and channel research in general.

Original languageEnglish
Pages (from-to)3151-3154
Number of pages4
JournalAnalytical Chemistry
Volume81
Issue number8
DOIs
Publication statusPublished - Apr 15 2009
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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