Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines.

Keiichiro Nakamura, Yutaka Yasunaga, Takehiko Segawa, Daejin Ko, Judd W. Moul, Shiv Srivastava, Johng S. Rhim

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Curcumin, traditionally used as a seasoning spice in Indian cuisine, has been reported to decrease the proliferation potential of prostate cancer cells, by a mechanism that is not fully understood. In the current study, we have evaluated the effects of curcumin in cell growth, activation of signal transduction, and transforming activities of both androgen-dependent and independent cell lines. Prostate cancer cell lines, LNCaP and PC-3, were treated with curcumin and its effects were further analyzed on signal transduction and expression of androgen receptor (AR) and AR-related cofactors using transient transfection assay and Western blotting. Our results show that curcumin down-regulates transactivation and expression of AR, activator protein-1 (AP-1), nuclear factor-kappaB (NF-kappaB), and CREB (cAMP response element-binding protein)-binding protein (CBP). Curcumin also inhibited the transforming activities of both cell lines as evidenced by the reduced colony forming ability in soft agar. The results obtained here demonstrate that curcumin has a potential therapeutic effect on prostate cancer cells through down-regulation of AR and AR-related cofactors (AP-1, NF-kappaB and CBP).

Original languageEnglish
Pages (from-to)825-830
Number of pages6
JournalInternational Journal of Oncology
Volume21
Issue number4
Publication statusPublished - Oct 2002
Externally publishedYes

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Curcumin
Androgen Receptors
Transcriptional Activation
Prostatic Neoplasms
Down-Regulation
Gene Expression
Cell Line
Transcription Factor AP-1
Signal Transduction
Cyclic AMP Response Element-Binding Protein
Spices
Therapeutic Uses
Protein Binding
Androgens
Agar
Transfection
Carrier Proteins
Western Blotting
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Nakamura, K., Yasunaga, Y., Segawa, T., Ko, D., Moul, J. W., Srivastava, S., & Rhim, J. S. (2002). Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. International Journal of Oncology, 21(4), 825-830.

Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. / Nakamura, Keiichiro; Yasunaga, Yutaka; Segawa, Takehiko; Ko, Daejin; Moul, Judd W.; Srivastava, Shiv; Rhim, Johng S.

In: International Journal of Oncology, Vol. 21, No. 4, 10.2002, p. 825-830.

Research output: Contribution to journalArticle

Nakamura, K, Yasunaga, Y, Segawa, T, Ko, D, Moul, JW, Srivastava, S & Rhim, JS 2002, 'Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines.', International Journal of Oncology, vol. 21, no. 4, pp. 825-830.
Nakamura, Keiichiro ; Yasunaga, Yutaka ; Segawa, Takehiko ; Ko, Daejin ; Moul, Judd W. ; Srivastava, Shiv ; Rhim, Johng S. / Curcumin down-regulates AR gene expression and activation in prostate cancer cell lines. In: International Journal of Oncology. 2002 ; Vol. 21, No. 4. pp. 825-830.
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