Cultured periodontal ligament fibroblasts express diverse connexins

Yuji Yamaoka, Yoshihiko Sawa, Noriyuki Ebata, Naoko Ibuki, Shigemitsu Yoshida

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Recent studies have suggested multiple functions of periodontal ligament fibroblasts (PDLFs) which may relate to the permeability of gap junctions composed of various types of connexins (Cxs). At present, 15 types of Cxs are known to exist, and six of their antibodies, anti-Cx26, Cx32, Cx37, Cx40, Cx43, and Cx45 are commercially available. This study aims to examine which types of Cxs are expressed in cultured PDLFs by an immunohistochemical method, western blotting, and RT-PCR. The study confirmed the expressions of Cx32, Cx40, Cx43, and Cx45 in PDLFs, while Cx26 and Cx37 were not detected. Considering previous reports, Cx32 may relate to the secretory function, and Cx40 and Cx45 to the contractile function of PDLFs, however, a function for Cx43 has not been specified. In the immunohistochemical examination, different localizations of Cx40/43 and Cx32/45 were established. The former were observed punctately, suggesting that a large part of Cx40/43 may exist in the cell membrane and construct gap junctions. In contrast, the latter were observed uniformly in all the cells, indicating that they are present both in the cell membrane and in the cytoplasm of the cells.

Original languageEnglish
Pages (from-to)375-380
Number of pages6
JournalTissue and Cell
Volume34
Issue number6
DOIs
Publication statusPublished - Dec 2002
Externally publishedYes

Keywords

  • Connexin
  • Fibroblast
  • Periodontal ligament

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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