Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis

Marie Okumura, Yoshiko Fujitani, Masahiko Maekawa, Jittima Charoenpanich, Hunja Murage, Kazuhide Kimbara, Nurettin Sahin, Akio Tani

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species–species specificity of plant–microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions.

Original languageEnglish
Pages (from-to)190-196
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume123
Issue number2
DOIs
Publication statusPublished - Feb 1 2017

Fingerprint

Methylobacterium
Biodiversity
Ionization
Seed
Desorption
Seeds
Lasers
Bacteria
Kenya
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Thailand
Growth
Farms
Mass spectrometry
Japan
Research
Oryza

Keywords

  • Methylobacterium species
  • Rice seeds
  • Whole-cell matrix-assisted laser desorption/ionization-mass spectrometry

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis. / Okumura, Marie; Fujitani, Yoshiko; Maekawa, Masahiko; Charoenpanich, Jittima; Murage, Hunja; Kimbara, Kazuhide; Sahin, Nurettin; Tani, Akio.

In: Journal of Bioscience and Bioengineering, Vol. 123, No. 2, 01.02.2017, p. 190-196.

Research output: Contribution to journalArticle

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