Abstract
Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species–species specificity of plant–microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions.
| Original language | English |
|---|---|
| Pages (from-to) | 190-196 |
| Number of pages | 7 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 123 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 1 2017 |
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Keywords
- Methylobacterium species
- Rice seeds
- Whole-cell matrix-assisted laser desorption/ionization-mass spectrometry
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
Cite this
Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis. / Okumura, Marie; Fujitani, Yoshiko; Maekawa, Masahiko; Charoenpanich, Jittima; Murage, Hunja; Kimbara, Kazuhide; Sahin, Nurettin; Tani, Akio.
In: Journal of Bioscience and Bioengineering, Vol. 123, No. 2, 01.02.2017, p. 190-196.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cultivable Methylobacterium species diversity in rice seeds identified with whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis
AU - Okumura, Marie
AU - Fujitani, Yoshiko
AU - Maekawa, Masahiko
AU - Charoenpanich, Jittima
AU - Murage, Hunja
AU - Kimbara, Kazuhide
AU - Sahin, Nurettin
AU - Tani, Akio
PY - 2017/2/1
Y1 - 2017/2/1
N2 - Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species–species specificity of plant–microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions.
AB - Methylobacterium species are methylotrophic bacteria that widely inhabit plant surfaces. In addition to studies on methylotrophs as model organisms, research has also been conducted on their mechanism of plant growth promotion as well as the species–species specificity of plant–microbe interaction. We employed whole-cell matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (WC-MS) analysis, which enables the rapid and accurate identification of bacteria at the species level, to identify Methylobacterium isolates collected from the rice seeds of different cultivars harvested in Japan, Thailand, and Kenya. Rice seeds obtained from diverse geographical locations showed different communities of Methylobacterium species. We found that M. fujisawaense, M. aquaticum, M. platani, and M. radiotolerans are the most frequently isolated species, but none were isolated as common species from 18 seed samples due to the highly biased communities in some samples. These findings will contribute to the development of formulations containing selected species that promote rice growth, though it may be necessary to customize the formulations depending on the cultivars and farm conditions.
KW - Methylobacterium species
KW - Rice seeds
KW - Whole-cell matrix-assisted laser desorption/ionization-mass spectrometry
UR - http://www.scopus.com/inward/record.url?scp=84992378755&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84992378755&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2016.09.001
DO - 10.1016/j.jbiosc.2016.09.001
M3 - Article
C2 - 27720675
AN - SCOPUS:84992378755
VL - 123
SP - 190
EP - 196
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 2
ER -