Cultivable butyrate-producing bacteria of elderly Japanese diagnosed with Alzheimer’s disease

Thi Thuy Tien Nguyen, Yuta Fujimura, Iyo Mimura, Yusuke Fujii, Ngoc Luong Nguyen, Kensuke Arakawa, Hidetoshi Morita

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The group of butyrate-producing bacteria within the human gut microbiome may be associated with positive effects on memory improvement, according to previous studies on dementia- associated diseases. Here, fecal samples of four elderly Japanese diagnosed with Alzheimer’s disease (AD) were used to isolate butyrate-producing bacteria. 226 isolates were randomly picked, their 16S rRNA genes were sequenced, and assigned into sixty OTUs (operational taxonomic units) based on BLASTn results. Four isolates with less than 97% homology to known sequences were considered as unique OTUs of potentially butyrate-producing bacteria. In addition, 12 potential butyrate-producing isolates were selected from the remaining 56 OTUs based on scan-searching against the PubMed and the ScienceDirect databases. Those belonged to the phylum Bacteroidetes and to the clostridial clusters I, IV, XI, XV, XIVa within the phylum Firmicutes. 15 out of the 16 isolates were indeed able to produce butyrate in culture as determined by high-performance liquid chromatography with UV detection. Furthermore, encoding genes for butyrate formation in these bacteria were identified by sequencing of degenerately primed PCR products and included the genes for butyrate kinase (buk), butyryl-CoA: acetate CoAtransferase (but), CoA-transferase-related, and propionate CoA-transferase. The results showed that eight isolates possessed buk, while five isolates possessed but. The CoA-transfer- related gene was identified as butyryl-CoA:4-hydroxybutyrate CoA transferase (4-hbt) in four strains. No strains contained the propionate CoA-transferase gene. The biochemical and butyrate-producing pathways analyses of butyrate producers presented in this study may help to characterize the butyrate-producing bacterial community in the gut of AD patients.

Original languageEnglish
JournalJournal of Microbiology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Butyrates
Alzheimer Disease
Bacteria
Genes
Coenzyme A-Transferases
Bacteroidetes
Microbiota
Coenzyme A
rRNA Genes
PubMed
Dementia
Acetates
High Pressure Liquid Chromatography
Databases
Polymerase Chain Reaction

Keywords

  • 16S rRNA gene sequencing
  • Alzheimer’s disease
  • butyrate-producing bacteria
  • gut microbiota
  • short-chain fatty acids

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Cultivable butyrate-producing bacteria of elderly Japanese diagnosed with Alzheimer’s disease. / Nguyen, Thi Thuy Tien; Fujimura, Yuta; Mimura, Iyo; Fujii, Yusuke; Nguyen, Ngoc Luong; Arakawa, Kensuke; Morita, Hidetoshi.

In: Journal of Microbiology, 01.01.2018.

Research output: Contribution to journalArticle

Nguyen, Thi Thuy Tien ; Fujimura, Yuta ; Mimura, Iyo ; Fujii, Yusuke ; Nguyen, Ngoc Luong ; Arakawa, Kensuke ; Morita, Hidetoshi. / Cultivable butyrate-producing bacteria of elderly Japanese diagnosed with Alzheimer’s disease. In: Journal of Microbiology. 2018.
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