CTGF/Hcs24 interacts with the cytoskeletal protein actin in chondrocytes

Gen Yosimichi, Satoshi Kubota, Takako Hattori, Takashi Nishida, Kumiko Nawachi, Tohru Nakanishi, Masafumi Kamada, Teruko Takano-Yamamoto, Masaharu Takigawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Connective tissue growth factor/hypertrophic chondrocyte-specific gene product 24 (CTGF/Hcs24) displays multiple functions in several types of mesenchymal cells, including the promotion of proliferation and differentiation of chondrocytes. Recently, the internalization and intracellular function of CTGF/Hcs24 were indicated as well. In this study, a binding protein for this factor was purified from the cytosolic fraction of human chondrosarcoma-derived chondrocytic cell line (HCS-2/8) by CTGF/Hcs24-affinity chromatography. The apparent molecular weight of the protein was 42 kDa and determination of the internal amino acid sequence revealed this protein to be β- or γ-actin. An in vitro competitive binding assay of 125I-labeled recombinant CTGF/Hcs24 with cold-rCTGF/Hcs24 showed that the binding between actin and 125I-CTGF/Hcs24 was specific. Immunoprecipitation analysis also showed that CTGF/Hcs24 bound to actin in HCS-2/8 cells. However, rCTGF/Hcs24 had no effects on the expression level of γ-actin mRNA or total actin protein. These findings suggest that a significant portion of intracellular CTGF/Hcs24 may regulate certain cell biological events in chondrocytes through the interaction with this particular cytoskeletal protein.

Original languageEnglish
Pages (from-to)755-761
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume299
Issue number5
DOIs
Publication statusPublished - Dec 1 2002

Keywords

  • Actin
  • Cell cycle
  • Chondrocyte
  • Connective tissue growth factor/hypertrophic chondrocyte-specific gene product
  • Cytoskeleton
  • Purification

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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